Literature DB >> 3003034

Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris.

C Li, H D Peck, A E Przybyla.   

Abstract

A PyrF- mutant of Escherichia coli (SK1108, pyrF::Tn5 Kanr) was complemented with the Desulfovibrio vulgaris (Hildenborough) structural gene for orotidine-5'-phosphate decarboxylase (EC 4.1.1.23). Either orientation of a 1.6-kilobase-pair D. vulgaris DNA fragment (pLP3B or pLP3A) complemented the PyrF- strain suggesting that the D. vulgaris pyrF promoter was functional. The apparent product of the D. vulgaris pyrF gene was a single 26-kilodalton polypeptide. These results demonstrate the utility of E. coli cloning systems in studying metabolic and energetic pathways in sulfate-reducing bacteria.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3003034      PMCID： PMC214468          DOI： 10.1128/jb.165.2.644-646.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  In vivo studies of temperature-sensitive recB and recC mutants.

Authors:  S R Kushner
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490

4.  Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.

Authors:  M Bazaral; D R Helinski
Journal:  J Mol Biol       Date:  1968-09-14       Impact factor: 5.469

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Biochemistry of dissimilatory sulphate reduction.

Authors:  H D Peck; J LeGall
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1982-09-13       Impact factor: 6.237

7.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

8.  Cloning and physical analysis of the pyrF gene (coding for orotidine-5'-phosphate decarboxylase) from Escherichia coli K-12.

Authors:  W P Donovan; S R Kushner
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

9.  The sulfur cycle.

Authors:  W W Kellogg; R D Cadle; E R Allen; A L Lazrus; E A Martell
Journal:  Science       Date:  1972-02-11       Impact factor: 47.728

10.  Cloning of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough) and determination of the NH2-terminal sequence.

Authors:  G Voordouw; J E Walker; S Brenner
Journal:  Eur J Biochem       Date:  1985-05-02
View more
  4 in total

1.  Genetic transfer in Desulfovibrio desulfuricans.

Authors:  B J Rapp; J D Wall
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

2.  Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibrio baculatus.

Authors:  N K Menon; H D Peck; J L Gall; A E Przybyla
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

3.  Cloning in Escherichia coli of genes involved in the synthesis of proline and leucine in Desulfovibrio desulfuricans Norway.

Authors:  M Fons; B Cami; J C Patte; M Chippaux
Journal:  Mol Gen Genet       Date:  1987-01

4.  Characterization of a small plasmid from Desulfovibrio desulfuricans and its use for shuttle vector construction.

Authors:  J D Wall; B J Rapp-Giles; M Rousset
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.