| Literature DB >> 3003024 |
W D Beck, B Berger-Bächi, F H Kayser.
Abstract
Additional DNA was shown to be present in methicillin-resistant Staphylococcus aureus by one- and two-dimensional restriction endonuclease analyses of the chromosomal DNA. A 3.5-kilobase Bg/II fragment, which was present in methicillin-resistant strains but not in the isogenic methicillin-sensitive parental strain, was cloned into newly constructed plasmid pWDB1 in Escherichia coli. Hybridization of this 3.5-kilobase Bg/II fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant (mec). In addition, the fragment carries a sequence that is present in some large staphylococcal plasmids, as well as in penicillinase plasmid pI524.Entities:
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Year: 1986 PMID： 3003024 PMCID： PMC214427 DOI： 10.1128/jb.165.2.373-378.1986
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490