Javier Aragón1, Simona Salerno2, Loredana De Bartolo3, Silvia Irusta4, Gracia Mendoza5. 1. Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain. Electronic address: jaragon@unizar.es. 2. Institute for Membrane Technology, National Research Council of Italy, ITM-CNR c/o University of Calabria, Via P. Bucci cubo 17/C, I-87036 Rende, Italy. Electronic address: s.salerno@itm.cnr.it. 3. Institute for Membrane Technology, National Research Council of Italy, ITM-CNR c/o University of Calabria, Via P. Bucci cubo 17/C, I-87036 Rende, Italy. Electronic address: l.debartolo@itm.cnr.it. 4. Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain; Networking Research Center for Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain. Electronic address: sirusta@unizar.es. 5. Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain. Electronic address: gmmenc@unizar.es.
Abstract
HYPOTHESIS: The development of novel scaffolds based on biocompatible polymers is of great interest in the field of bone repair for fabrication of biodegradable scaffolds that mimic the extracellular matrix and have osteoconductive and osteoinductive properties for enhanced bone regeneration. EXPERIMENTS: Polycaprolactone (PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) core-shell fibers were synthesised and decorated with poly(lactic-co-glycolic acid) [PLGA] particles loaded with bone morphogenetic protein 2 (BMP2) by simultaneous electrospinning and electrospraying. Hydroxyapatite nanorods (HAn) were loaded into the core of fibers. The obtained scaffolds were characterised by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The in vitro potential of these materials for bone regeneration was assessed in biodegradation assays, osteoblast viability assays, and analyses of expression of specific bone markers, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). FINDINGS: PLGA particles were homogeneously distributed in the entire fibre mat. The growth factor load was 1.2-1.7 μg/g of the scaffold whereas the HAn load was in the 8.8-12.6 wt% range. These scaffolds were able to support and enhance cell growth and proliferation facilitating the expression of osteogenic and osteoconductive markers (OCN and OPN). These observations underline the great importance of the presence of BMP2 in scaffolds for bone remodelling as well as the good potential of the newly developed scaffolds for clinical use in tissue engineering.
HYPOTHESIS: The development of novel scaffolds based on biocompatible polymers is of great interest in the field of bone repair for fabrication of biodegradable scaffolds that mimic the extracellular matrix and have osteoconductive and osteoinductive properties for enhanced bone regeneration. EXPERIMENTS: Polycaprolactone (PCL) and polycaprolactone/polyvinyl acetate (PCL/PVAc) core-shell fibers were synthesised and decorated with poly(lactic-co-glycolic acid) [PLGA] particles loaded with bone morphogenetic protein 2 (BMP2) by simultaneous electrospinning and electrospraying. Hydroxyapatite nanorods (HAn) were loaded into the core of fibers. The obtained scaffolds were characterised by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The in vitro potential of these materials for bone regeneration was assessed in biodegradation assays, osteoblast viability assays, and analyses of expression of specific bone markers, such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). FINDINGS: PLGA particles were homogeneously distributed in the entire fibre mat. The growth factor load was 1.2-1.7 μg/g of the scaffold whereas the HAn load was in the 8.8-12.6 wt% range. These scaffolds were able to support and enhance cell growth and proliferation facilitating the expression of osteogenic and osteoconductive markers (OCN and OPN). These observations underline the great importance of the presence of BMP2 in scaffolds for bone remodelling as well as the good potential of the newly developed scaffolds for clinical use in tissue engineering.
Authors: Zeynep Aytac; Nileshkumar Dubey; Arwa Daghrery; Jessica A Ferreira; Isaac J de Souza Araújo; Miguel Castilho; Jos Malda; Marco C Bottino Journal: Int Mater Rev Date: 2021-07-05 Impact factor: 15.750
Authors: Małgorzata Krok-Borkowicz; Katarzyna Reczyńska; Łucja Rumian; Elżbieta Menaszek; Maciej Orzelski; Piotr Malisz; Piotr Silmanowicz; Piotr Dobrzyński; Elżbieta Pamuła Journal: Int J Mol Sci Date: 2020-10-13 Impact factor: 5.923