Ursula Oestreicher1, David Endesfelder1, Maria Gomolka1, Ausrele Kesminiene2, Peter Lang3, Carita Lindholm4, Ute Rößler1, Daniel Samaga1,5, Ulrike Kulka1. 1. a Federal Office for Radiation Protection (BfS) , Oberschleissheim , Germany. 2. b International Agency for Research on Cancer, IARC , Lyon , France. 3. c Department of Radiation Oncology , University Hospital, LMU , Munich , Germany. 4. d Radiation and Nuclear Safety Authority, STUK , Helsinki , Finland. 5. e Research Unit Radiation Cytogenetics , Helmholtz Zentrum Muenchen , Oberschleissheim , Germany.
Abstract
PURPOSE: Automated detection of dicentric chromosomes from a large number of cells was applied to study age-dependent radiosensitivity after in vitro CT exposure of blood from healthy donors. MATERIALS AND METHODS: Blood samples from newborns, children (2-5 years) and adults (20-50 years) were exposed in vitro to 0 mGy, 41 mGy and 978 mGy using a CT equipment. In this study, automated scoring based on 13,000-31,000 cells/dose point/age group was performed. Results for control and low dose points were validated by manually counting about 26,000 cells/dose point/age group. RESULTS: For all age groups, the high number of analyzed cells enabled the detection of a significant increase in the frequency of radiation induced dicentric chromosomes in cells exposed to 41 mGy as compared to control cells. Moreover, differences between the age groups could be resolved for the low dose: young donors showed significantly increased risk for induced dicentrics at 41 mGy compared to adults. CONCLUSIONS: The results very clearly demonstrate that the automated dicentric scoring method is capable of discerning radiation induced biomarkers in the low dose range (<100 mGy) and thus may open possibilities for large-scale molecular epidemiology studies in radiation protection.
PURPOSE: Automated detection of dicentric chromosomes from a large number of cells was applied to study age-dependent radiosensitivity after in vitro CT exposure of blood from healthy donors. MATERIALS AND METHODS: Blood samples from newborns, children (2-5 years) and adults (20-50 years) were exposed in vitro to 0 mGy, 41 mGy and 978 mGy using a CT equipment. In this study, automated scoring based on 13,000-31,000 cells/dose point/age group was performed. Results for control and low dose points were validated by manually counting about 26,000 cells/dose point/age group. RESULTS: For all age groups, the high number of analyzed cells enabled the detection of a significant increase in the frequency of radiation induced dicentric chromosomes in cells exposed to 41 mGy as compared to control cells. Moreover, differences between the age groups could be resolved for the low dose: young donors showed significantly increased risk for induced dicentrics at 41 mGy compared to adults. CONCLUSIONS: The results very clearly demonstrate that the automated dicentric scoring method is capable of discerning radiation induced biomarkers in the low dose range (<100 mGy) and thus may open possibilities for large-scale molecular epidemiology studies in radiation protection.
Authors: Mark P Little; Richard Wakeford; Simon D Bouffler; Kossi Abalo; Michael Hauptmann; Nobuyuki Hamada; Gerald M Kendall Journal: Environ Int Date: 2021-12-24 Impact factor: 13.352