Brent K Larson1, Richard Tuli2, Laith H Jamil3, Simon K Lo3, Nan Deng4, Andrew E Hendifar3. 1. From the Department of Pathology and Laboratory Medicine. 2. Department of Radiation Oncology. 3. Department of Medicine, and. 4. Biostatistics and Bioinformatics Research Center, Cedars-Sinai Medical Center, Los Angeles, CA.
Abstract
OBJECTIVES: Endoscopic ultrasound-guided fine-needle biopsy and aspiration (EUS FNB/A) are the standard diagnostic tests for pancreatic malignancies. Oncologists increasingly use tumor genomic analysis for management. Adequacy of FNB/A tissue for next-generation sequencing (NGS) has not been evaluated. This study examined FNB/A and other biopsy types for features that might predict adequacy for NGS. METHODS: Seventy-six pancreatic exocrine malignancy biopsies submitted for NGS were assessed for adequacy, which was compared with other tumor/biopsy features. RESULTS: Twenty-two (29%) of 76 samples were inadequate, including 16 (30%) of 54 FNBs and 4 (57%) of 7 FNAs. Larger-gauge needles were associated with adequacy in all samples (P = 0.0047) and in FNBs (P = 0.05). Metastatic samples were more likely to be adequate for NGS compared with pancreatic samples (P = 0.0357). Percutaneous biopsies were more likely to be adequate than EUS-guided FNB/As, although this trend was not significant (P = 0.0558). Other tumor/biopsy characteristics were not associated with adequacy. CONCLUSIONS: Endoscopic US FNA and FNB provided similar NGS adequacy rates. Metastatic lesions accessible by percutaneous biopsy may be preferable to EUS FNB/A of primary lesions for obtaining tissue for NGS. All biopsies, including EUS FNB, were more likely to be successful using larger-gauge needles.
OBJECTIVES: Endoscopic ultrasound-guided fine-needle biopsy and aspiration (EUS FNB/A) are the standard diagnostic tests for pancreatic malignancies. Oncologists increasingly use tumor genomic analysis for management. Adequacy of FNB/A tissue for next-generation sequencing (NGS) has not been evaluated. This study examined FNB/A and other biopsy types for features that might predict adequacy for NGS. METHODS: Seventy-six pancreatic exocrine malignancy biopsies submitted for NGS were assessed for adequacy, which was compared with other tumor/biopsy features. RESULTS: Twenty-two (29%) of 76 samples were inadequate, including 16 (30%) of 54 FNBs and 4 (57%) of 7 FNAs. Larger-gauge needles were associated with adequacy in all samples (P = 0.0047) and in FNBs (P = 0.05). Metastatic samples were more likely to be adequate for NGS compared with pancreatic samples (P = 0.0357). Percutaneous biopsies were more likely to be adequate than EUS-guided FNB/As, although this trend was not significant (P = 0.0558). Other tumor/biopsy characteristics were not associated with adequacy. CONCLUSIONS: Endoscopic US FNA and FNB provided similar NGS adequacy rates. Metastatic lesions accessible by percutaneous biopsy may be preferable to EUS FNB/A of primary lesions for obtaining tissue for NGS. All biopsies, including EUS FNB, were more likely to be successful using larger-gauge needles.
Authors: Sherif Elhanafi; Nadim Mahmud; Norge Vergara; Michael L Kochman; Koushik K Das; Gregory G Ginsberg; Michael Rajala; Vinay Chandrasekhara Journal: J Gastroenterol Hepatol Date: 2018-12-10 Impact factor: 4.369
Authors: Priscilla A van Riet; Djuna L Cahen; Katharina Biermann; Bettina Hansen; Alberto Larghi; Guido Rindi; Giovanni Fellegara; Paolo Arcidiacono; Claudio Doglioni; Nicola Liberta Decarli; Julio Iglesias-Garcia; Ihab Abdulkader; Hector Lazare Iglesias; Masayuki Kitano; Takaaki Chikugo; Satoru Yasukawa; Hans van der Valk; Nam Quoc Nguyen; Andrew Ruszkiewicz; Marc Giovannini; Flora Poizat; Schalk van der Merwe; Tania Roskams; Erwin Santo; Silvia Marmor; Kenneth Chang; Fritz Lin; James Farrell; Marie Robert; Juan Carlos Bucobo; Alan Heimann; Francisco Baldaque-Silva; Carlos Fernández Moro; Marco J Bruno Journal: Dig Endosc Date: 2019-07-10 Impact factor: 7.559
Authors: Joseph R Habib; Yayun Zhu; Lingdi Yin; Ammar A Javed; Ding Ding; Jonathan Tenior; Michael Wright; Syed Z Ali; Richard A Burkhart; William Burns; Christopher L Wolfgang; Eunji Shin; Jun Yu; Jin He Journal: J Gastrointest Surg Date: 2021-07-09 Impact factor: 3.452