Juhee Shin1, Yuhua Yin1, Hyewon Park1, Seungjo Park2, Ursula L Triantafillu2, Yonghyun Kim2, Sang Ryong Kim3, Sun Yeul Lee4, Do Kyung Kim5, Jinpyo Hong6, Dong Woon Kim1,6. 1. Department of Medical Science, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea. 2. Department of Chemical & Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA. 3. BK21 plus KNU Creative BioResearch Group, School of Life Sciences, Institute of Life Science & Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea. 4. Department of Anesthesia & Pain Medicine, Chungnam National University Hospital, Daejeon 35015, Republic of Korea. 5. Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea. 6. Department of Anatomy, Brain Research Institute, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea.
Abstract
AIM: To investigate whether p38 small-interfering RNA-loaded nanoparticles (p38 siRNA NPs) attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats by suppressing spinal microglia activation via p38 targeting. MATERIALS & METHODS: After synthesizing p38 siRNA NPs with sonication, physical characteristics were measured for size and zeta potential. p38 siRNA NPs were then administrated intrathecally into SNL rats if they could reduce pain behavior excellently. RESULTS: p38 siRNA NPs significantly reduced mechanical allodynia as well as microgliosis in the spinal dorsal horns of SNL rats, consistent with a downregulation of p38-related proinflammatory mediators. CONCLUSION: As p38 in the spinal microglia plays a critical role in neuropathic pain, we expect that p38 siRNA NPs could be a promising tool for the treatment of neuropathic pain.
AIM: To investigate whether p38 small-interfering RNA-loaded nanoparticles (p38 siRNA NPs) attenuate spinal nerve ligation (SNL)-induced neuropathic pain in rats by suppressing spinal microglia activation via p38 targeting. MATERIALS & METHODS: After synthesizing p38 siRNA NPs with sonication, physical characteristics were measured for size and zeta potential. p38 siRNA NPs were then administrated intrathecally into SNL rats if they could reduce pain behavior excellently. RESULTS:p38 siRNA NPs significantly reduced mechanical allodynia as well as microgliosis in the spinal dorsal horns of SNL rats, consistent with a downregulation of p38-related proinflammatory mediators. CONCLUSION: As p38 in the spinal microglia plays a critical role in neuropathic pain, we expect that p38 siRNA NPs could be a promising tool for the treatment of neuropathic pain.
Authors: Chan Noh; Hyo Jung Shin; Seounghun Lee; Song I Kim; Yoon-Hee Kim; Won Hyung Lee; Dong Woon Kim; Sun Yeul Lee; Young Kwon Ko Journal: Int J Mol Sci Date: 2020-05-14 Impact factor: 5.923