Mariana Séfora Bezerra Sousa1, Iasmym Melissa Soares de Holanda2, Heloísa Mirelle Costa Monteiro1, Ângela Amâncio-Dos-Santos3. 1. Fellow PhD degree, Postgraduate Program in Nutrition, Department of Nutrition, Center for Health Sciences, Universidade Federal de Pernambuco (UFPE), Recife-PE, Brazil. Intellectual, scientific, conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript writing; final approval. 2. Graduate student, Department of Physiology and Pharmacology, Biosciences Center, UFPE, Recife-PE, Brazil. Acquisition of data, technical procedures. 3. PhD, Associate Professor, Department of Physiology and Pharmacology, Biosciences Center, UFPE, Recife-PE, Brazil. Intellectual, scientific, conception and design of the study; acquisition, analysis and interpretation of data; statistical analysis; manuscript writing; critical revision; final approval.
Abstract
PURPOSE: To investigate the effects of Murici extract on the brain excitability-dependent phenomenon known as cortical spreading depression (CSD) and on brain oxidative stress. METHODS: Adult and aged Wistar rats were supplemented with murici extract (150 mg/kg/day or 300 mg/kg/day) by gavage for fifteen days. Afterwards, the animals were submitted to a CSD electrophysiological recording and to brain oxidative stress evaluation. RESULTS: Our results showed that aging decreased CSD propagation velocity, catalase activity and glutathione/oxidized glutathione ratio (GSH/GSSG) in the brain cortex of the rats, and increased malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activity. The highest dose (300 mg/kg/day) of murici extract accelerated CSD, whereas the lowest (150mg/kg/day) decelerated, in both adult and aged animals. In contrast, aged animals supplemented with murici extract in both doses presented low MDA levels and high GSG/GSSG ratio in comparison to the control-aged animals. CONCLUSION: Murici extract supplementation seems to revert detrimental effects in aged brains and could be considered as a strategy in the treatment of pathologies related to aging and cortical spreading depression.
PURPOSE: To investigate the effects of Murici extract on the brain excitability-dependent phenomenon known as cortical spreading depression (CSD) and on brain oxidative stress. METHODS: Adult and aged Wistar rats were supplemented with murici extract (150 mg/kg/day or 300 mg/kg/day) by gavage for fifteen days. Afterwards, the animals were submitted to a CSD electrophysiological recording and to brain oxidative stress evaluation. RESULTS: Our results showed that aging decreased CSD propagation velocity, catalase activity and glutathione/oxidized glutathione ratio (GSH/GSSG) in the brain cortex of the rats, and increased malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activity. The highest dose (300 mg/kg/day) of murici extract accelerated CSD, whereas the lowest (150mg/kg/day) decelerated, in both adult and aged animals. In contrast, aged animals supplemented with murici extract in both doses presented low MDA levels and high GSG/GSSG ratio in comparison to the control-aged animals. CONCLUSION:Murici extract supplementation seems to revert detrimental effects in aged brains and could be considered as a strategy in the treatment of pathologies related to aging and cortical spreading depression.
Authors: E M S Silveira; M C Q Santos; T C B da Silva; F B O Silva; C V Machado; L Elias; A Kolberg; A Kroth; W A Partata Journal: Braz J Med Biol Res Date: 2020-05-08 Impact factor: 2.590