Mario Musella1, Francesco Di Capua2, Maria D'Armiento3, Nunzio Velotti3, Alessio Bocchetti3, Katia Di Lauro3, Giuseppe Galloro4, Severo Campione3, Giuseppe Petrella5, Francesco Paolo D'Armiento3. 1. Advanced Biomedical Sciences Department, Naples "Federico II" University, AOU "Federico II" - Via S. Pansini 5 Building 12, 80131, Naples, Italy. mario.musella@unina.it. 2. Cardiothoracic and Respiratory Sciences Department, University of Campania "Luigi Vanvitelli", Caserta, Italy. 3. Advanced Biomedical Sciences Department, Naples "Federico II" University, AOU "Federico II" - Via S. Pansini 5 Building 12, 80131, Naples, Italy. 4. Clinical Medicine and Surgery Department, Naples "Federico II" University, Naples, Italy. 5. General Surgery Department, University of Rome "Tor Vergata", Rome, Italy.
Abstract
BACKGROUND: To understand the role of ghrelin in the mechanism of action of laparoscopic sleeve gastrectomy (LSG), a prospective cohort case-control study to assess the expression of ghrelin-producing cells (GPC) in two groups of patients was designed. METHODS: Specimens of resected stomach from 26 obese patients who underwent LSG (group A), were compared by immunohistochemistry to control stomach samples from 26 non-obese patients (group B) resected for other pathologies or during autopsy; (GIST: 6 cases, inflammatory diseases: 4 cases, post-mortem autopsy cases with stomachs from healthy persons victims of traumatic accidents: 16 cases). Immunohistochemistry investigation was performed with the use of Ventana Benchmark ultra, anti-ghrelin antibody NOVUS, mouse monoclonal 2F4, diluted at 1:100. RESULTS: No significant difference in the expression of GPC number between group A and B was found (p = 0.87). No significant correlation between patients presenting a GPC number above (subgroup 1) or below (subgroup 2) the average, and EWL% changes, both at 1 and 6 years of follow-up, was recorded. CONCLUSIONS: Our study has shown that the expression of GPC is similar in the stomach of obese and non-obese controls, being mostly influenced by the inflammatory status of the gastric mucosa. A variation in the preoperative number of GPC has not influenced the weight loss in patients who underwent LSG.
BACKGROUND: To understand the role of ghrelin in the mechanism of action of laparoscopic sleeve gastrectomy (LSG), a prospective cohort case-control study to assess the expression of ghrelin-producing cells (GPC) in two groups of patients was designed. METHODS: Specimens of resected stomach from 26 obesepatients who underwent LSG (group A), were compared by immunohistochemistry to control stomach samples from 26 non-obesepatients (group B) resected for other pathologies or during autopsy; (GIST: 6 cases, inflammatory diseases: 4 cases, post-mortem autopsy cases with stomachs from healthy persons victims of traumatic accidents: 16 cases). Immunohistochemistry investigation was performed with the use of Ventana Benchmark ultra, anti-ghrelin antibody NOVUS, mouse monoclonal 2F4, diluted at 1:100. RESULTS: No significant difference in the expression of GPC number between group A and B was found (p = 0.87). No significant correlation between patients presenting a GPC number above (subgroup 1) or below (subgroup 2) the average, and EWL% changes, both at 1 and 6 years of follow-up, was recorded. CONCLUSIONS: Our study has shown that the expression of GPC is similar in the stomach of obese and non-obese controls, being mostly influenced by the inflammatory status of the gastric mucosa. A variation in the preoperative number of GPC has not influenced the weight loss in patients who underwent LSG.
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Authors: M Musella; M Milone; D Gaudioso; P Bianco; R Palumbo; G Galloro; M Bellini; F Milone Journal: Int J Surg Date: 2014-05-23 Impact factor: 6.071
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Authors: Adam P Chambers; Henriette Kirchner; Hilary E Wilson-Perez; Jill A Willency; John E Hale; Bruce D Gaylinn; Michael O Thorner; Paul T Pfluger; Jesus A Gutierrez; Matthias H Tschöp; Darleen A Sandoval; Randy J Seeley Journal: Gastroenterology Date: 2012-09-17 Impact factor: 22.682
Authors: John E Farey; Tamara C Preda; Oliver M Fisher; Angelique J Levert-Mignon; Rebecca L Stewart; Elisabeth Karsten; Benjamin R Herbert; Michael M Swarbrick; Reginald V Lord Journal: Obes Surg Date: 2017-02 Impact factor: 4.129
Authors: N Velotti; M Manigrasso; K Di Lauro; A Vitiello; G Berardi; D Manzolillo; P Anoldo; A Bocchetti; F Milone; M Milone; G D De Palma; M Musella Journal: J Obes Date: 2019-01-03