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Literature DB >> 30019004

Quantification of Starch in Guard Cells of Arabidopsis thaliana.

Sabrina Flütsch¹, Luca Distefano¹, Diana Santelia¹.

Abstract

In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.

Entities: Chemical

Keywords: Arabidopsis; Guard cells; Propidium iodide; Starch; Stomatal opening

Year: 2018 PMID： 30019004 PMCID： PMC6046211 DOI： 10.21769/BioProtoc.2920

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

3 in total

1. High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis.

Authors: Elisabeth Truernit; Hélène Bauby; Bertrand Dubreucq; Olivier Grandjean; John Runions; Julien Barthélémy; Jean-Christophe Palauqui
Journal: Plant Cell Date: 2008-06-03 Impact factor: 11.277

Review 2. Transitory Starch Metabolism in Guard Cells: Unique Features for a Unique Function.

Authors: Diana Santelia; John E Lunn
Journal: Plant Physiol Date: 2017-03-14 Impact factor: 8.340

3. Blue Light Induces a Distinct Starch Degradation Pathway in Guard Cells for Stomatal Opening.

Authors: Daniel Horrer; Sabrina Flütsch; Diana Pazmino; Jack S A Matthews; Matthias Thalmann; Arianna Nigro; Nathalie Leonhardt; Tracy Lawson; Diana Santelia
Journal: Curr Biol Date: 2016-01-07 Impact factor: 10.834

3 in total

5 in total

1. Brassinosteroid and Hydrogen Peroxide Interdependently Induce Stomatal Opening by Promoting Guard Cell Starch Degradation.

Authors: Jin-Ge Li; Min Fan; Wenbo Hua; Yanchen Tian; Lian-Ge Chen; Yu Sun; Ming-Yi Bai
Journal: Plant Cell Date: 2020-02-12 Impact factor: 11.277

Quantification of Starch in Guard Cells of Arabidopsis thaliana.

1. High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis.

Review 2. Transitory Starch Metabolism in Guard Cells: Unique Features for a Unique Function.

3. Blue Light Induces a Distinct Starch Degradation Pathway in Guard Cells for Stomatal Opening.

1. Brassinosteroid and Hydrogen Peroxide Interdependently Induce Stomatal Opening by Promoting Guard Cell Starch Degradation.

2. Starch biosynthesis in guard cells has features of both autotrophic and heterotrophic tissues.

3. Glucose uptake to guard cells via STP transporters provides carbon sources for stomatal opening and plant growth.

4. Guard Cell Starch Degradation Yields Glucose for Rapid Stomatal Opening in Arabidopsis.

5. Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening.