The effect of ozone on hypoxia, hemolysis and morphological change of blood from patients with aortic dissection (AD): a preliminary in vitro experiment of ozonated autohemotherapy for treating AD.

This study aimed to investigate the effect of ozone on hypoxia, hemolysis and morphological change of blood from aortic dissection (AD) patients for providing preliminary evidence of application of ozonated autohemotherapy (Ozone-AHT) in AD patients. 20 AD patients and 20 healthy volunteers were consecutively included, and blood samples were collected from all participants and ozonized in vitro. PO2, SO2, malondialdehyde (MDA), superoxide dismutase (SOD), malformation percentage, morphology change and spatial distribution of filamentous actin (F-actin) in erythrocytes at different ozone concentrations were evaluated. After ozonation of whole blood, the median levels of PO2 and SO2 increased under Ozone concentrations at 40 μg/mL, 80 μg/mL and 160 μg/mL compared with samples exposed to 0 μg/mL in both AD group and control group. The MDA level was similar in samples exposed to 0 μg/mL, 40 μg/mL and 80 μg/mL ozone, while the levels of SOD increased in samples exposed to 40 μg/mL and 80 μg/mL in both AD group and control group. Compared with the samples exposed to 0 μg/mL ozone, FHb level only increased in samples exposed to 80 μg/mL and 160 μg/mL Ozone in both AD group and control group. In addition, overdosed ozone (160 μg/mL) but not therapeutic ozone concentrations (0 μg/mL, 40 μg/mL and 80 μg/mL) increased malformation percentage and morphology change of erythrocytes in both AD group and control group. In conclusion, Ozone improves oxygen content and reduces oxidative damage in blood from AD patients, and therapeutic dose ozone do not induce hemolysis and morphology change of erythrocytes.