Feng Zhou1,2, Dahui Ma1, Ming Ouyang1, Guiqin Liu1, Tianhui Zhu1, Yun Yang2. 1. Shenzhen Eye Hospital, Shenzhen Eye Centre, Jinan University, Shenzhen Key Laboratory of Ophthalmology Shenzhen, Guangdong Province, China. 2. Department of Ophthalmology, Puai Hospital Affiliated to Tongji Medical College of Huazhong Science and Technology University Wuhan, Hubei, China.
Abstract
OBJECTIVE: Mesenchymal stem cells (MSCs) derived from nasal mucosa are featured by high division and differentiation capacity, with large nuclei, obvious nucleoli and weak cytoplasmic basophily. Imaging examination, typically CT scan, is the gold standard for the diagnosis of orbital fracture. METHODS: We isolated MSCs derived from goat nasal mucosa and built the calcification model so as to investigate the repair mechanism of nasal mucosa-derived MSCs in orbital fracture. Expressions of osteogenic markers Runx2, OCN, OPN and BSP were detected using western blot. RESULTS: Nasal mucosa-derived MSCs were successfully isolated and passaged. Nestin was detected by immunofluorescence assay in the cells of the third generation. It was further confirmed that the isolated cells were nasal mucosa-derived MSCs. As indicated by alizarin red staining, the calcification model in nasal mucosa-derived MSCs was successfully built. The relative expressions of Runx2 and OCN reached the highest level after osteogenic induction for 7 d, and the expressions of OPN and BSP were also high. But at 10 d, the expressions of all markers declined somewhat. At 14 d, the expressions of OPN and BSP reached the peak, but without significant differences compared with those at 7 d. CONCLUSION: The present study suggested that the repair effect of nasal mucosa-derived MSCs in orbital fracture is achieved by facilitating the expressions of osteogenic markers Runx2, OCN, OPN and BSP. However, the pathways of actions are unknown and further studies are required to elucidate the concrete mechanism.
OBJECTIVE: Mesenchymal stem cells (MSCs) derived from nasal mucosa are featured by high division and differentiation capacity, with large nuclei, obvious nucleoli and weak cytoplasmic basophily. Imaging examination, typically CT scan, is the gold standard for the diagnosis of orbital fracture. METHODS: We isolated MSCs derived from goat nasal mucosa and built the calcification model so as to investigate the repair mechanism of nasal mucosa-derived MSCs in orbital fracture. Expressions of osteogenic markers Runx2, OCN, OPN and BSP were detected using western blot. RESULTS: Nasal mucosa-derived MSCs were successfully isolated and passaged. Nestin was detected by immunofluorescence assay in the cells of the third generation. It was further confirmed that the isolated cells were nasal mucosa-derived MSCs. As indicated by alizarin red staining, the calcification model in nasal mucosa-derived MSCs was successfully built. The relative expressions of Runx2 and OCN reached the highest level after osteogenic induction for 7 d, and the expressions of OPN and BSP were also high. But at 10 d, the expressions of all markers declined somewhat. At 14 d, the expressions of OPN and BSP reached the peak, but without significant differences compared with those at 7 d. CONCLUSION: The present study suggested that the repair effect of nasal mucosa-derived MSCs in orbital fracture is achieved by facilitating the expressions of osteogenic markers Runx2, OCN, OPN and BSP. However, the pathways of actions are unknown and further studies are required to elucidate the concrete mechanism.
Entities:
Keywords:
Nasal mucosa-derived mesenchymal stem cells; Western blotting; orbital fracture repair
Authors: Hamad Ebrahim Al Ahmed; Mohamed A Jaber; Salem H Abu Fanas; Mark Karas Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 2004-08