| Literature DB >> 30017848 |
Junjun Shang1, Yan Li3, Ruiheng Yang1, Ying Wang1, Wenjun Mao1, Lihua Tang1, Yingying Wu1, Takehito Nakazawa2, Yoichi Honda2, Yan Li3, Dapeng Bao4.
Abstract
We introduced a site-directed mutation in the sdi1 gene and used it as a selective marker for the polyethylene glycol-mediated transformation of Pleurotus eryngii monokaryon protoplasts. The transformants displayed obvious and stable resistance to the fungicide carboxin indicating that the mutant Pesdi1 gene is an efficient selective marker.Entities:
Keywords: Carboxin; Pleurotus eryngii; Selective marker; Transformation
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Year: 2018 PMID： 30017848 DOI： 10.1016/j.mimet.2018.07.006
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363