| Literature DB >> 30017081 |
Amir Abdoli1, Mohsen Arbabi2, Majid Pirestani3, Mehran Mirzaghavami3, Fatemeh Ghaffarifar3, Abdolhossein Dalimi3, Javid Sadraei3.
Abstract
Neospora caninum and Toxoplasma gondii are two closely related protozoan parasites that have been detected from various species of bird hosts. However, little is known about the prevalence of N. caninum and T. gondii in crows. Hence, we examined the molecular frequency of N. caninum and T. gondii in the brain samples of hooded crows (Corvus cornix) that collected from different public parks of Tehran, Iran by nested-PCR method. We used the primers targeting the Nc5 and GRA6 genes for detection of N. caninum and T. gondii, respectively. From a total of 55 brain samples, 5 (9.9%) and 9 (16.36%) samples were positive for N. caninum and T. gondii, respectively. Sequencing of a N. caninum isolate revealed 95%-100% identity with the deposited N. caninum in GenBank. Genotyping of T. gondii isolates by PCR-RFLP analysis of the GRA6 gene revealed type III genotype in 8 isolates. The results of this study indicate that hooded crows may have a putative role in transmission of N. caninum and T. gondii to canines and felines definitive hosts, respectively.Entities:
Keywords: Corvid; Corvus cornix; Iran; Neosporosis; PCR; Toxoplasmosis
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Year: 2018 PMID： 30017081 DOI： 10.1016/j.cimid.2018.06.008
Source DB: PubMed Journal: Comp Immunol Microbiol Infect Dis ISSN： 0147-9571 Impact factor: 2.268