Detection of Aujeszky's disease virus DNA and antibody in swine oral fluid specimens.

Aujeszky's disease virus (ADV) continues to circulate in commercial swine populations in many regions and in feral swine populations in most parts of the world, that is, ADV continues to present a risk to pork producers everywhere. Current DIVA vaccines and assays are highly effective in the control and/or eradication of ADV, but detection of wild-type ADV infection relies on testing individual pig specimens, for example, serum or muscle exudate ("meat juice"). Oral fluid specimens have been shown to be highly effective for the surveillance of a variety of swine pathogens and could offer the means to improve the efficiency of ADV surveillance in the field. In this study, the temporal patterns of ADV DNA and antibody detection in oral fluid and serum specimens were established in ADV-inoculated pigs (n = 14) using gB and gE PCRs, virus neutralization (VN) and three commercial serum antibody ELISAs (gB bELISA, gI bELISA and ADV iELISA). ADV DNA was detected in oral fluid samples (20% to 100%) from 3 to 21 days postinoculation (DPI), but not in serum. ADV antibody was detected in oral fluid specimens at DPI ≥ 10 with the gB bELISA (36% to 79%) and ADV iELISA (29% to 100%), but not the gI bELISA. These results suggest that oral fluid could be used as an alternative to individual pig sampling for ADV surveillance using PCR- and/or antibody-based assays.