Shi-Chao Wang 1,2 , Hui-Hui Fu 2 , Ji-Rui Wen 2 , Zhi-Wei Zhao 2 , Yong-Mei Nie 1 , Ya-Li Miao 3 , Jiang Wu 1,2 Show Affiliations »
Abstract
OBJECTIVE: To explore the effect of IL-8 on the epithelial-to-mesenchymal transition (EMT) in ovarian cancer,which will provide experimental basis for revealing related molecular mechanism in malignant metastasis of ovarian cancer. METHODS: The migration of ovarian cancer cell line SKOV3 cells was explored with Real time label free cell analysis (RTCA) after treatment with recombinant human IL-8.SKOV3 cells were co-cultured with IL-8 for 48 h,proteins involved in EMT were investigated via Western blot to explore the effect of IL-8 on the activation of the EMT. Invasion of SKOV3 cells after treatment with IL-8 were evaluated by transwell assay. RESULTS: According to the results of RTCA,after treatment with IL-8 for 48 h,the migration of SKOV3 cells was in platform phase. The treatment of IL-8 unregulated vimentin and snail and downregulated E-cadherin,which suggested that IL-8 induced EMT in ovarian cancer. The results of transwell test showed that invasive ability of IL-8 pretreated SKOV3 cells was enhanced (P<0.05). CONCLUSION: IL-8 can induce the EMT of ovarian cancer and enhance the invasion and migration of ovarian cancer. Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).
OBJECTIVE: To explore the effect of IL-8 on the epithelial-to-mesenchymal transition (EMT) in ovarian cancer ,which will provide experimental basis for revealing related molecular mechanism in malignant metastasis of ovarian cancer . METHODS: The migration of ovarian cancer cell line SKOV3 cells was explored with Real time label free cell analysis (RTCA ) after treatment with recombinant human IL-8 .SKOV3 cells were co-cultured with IL-8 for 48 h,proteins involved in EMT were investigated via Western blot to explore the effect of IL-8 on the activation of the EMT. Invasion of SKOV3 cells after treatment with IL-8 were evaluated by transwell assay. RESULTS: According to the results of RTCA ,after treatment with IL-8 for 48 h,the migration of SKOV3 cells was in platform phase. The treatment of IL-8 unregulated vimentin and snail and downregulated E-cadherin ,which suggested that IL-8 induced EMT in ovarian cancer . The results of transwell test showed that invasive ability of IL-8 pretreated SKOV3 cells was enhanced (P<0.05). CONCLUSION: IL-8 can induce the EMT of ovarian cancer and enhance the invasion and migration of ovarian cancer . Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).
Entities: Disease
Gene
Species
Keywords:
Epithelial-to-mesenchymal transitionzzm321990; IL-8zzm321990; Ovarian cancerzzm321990
Mesh： See more »
Substances： See more »
Year: 2018
PMID： 30014646
Source DB: PubMed Journal: Sichuan Da Xue Xue Bao Yi Xue Ban ISSN： 1672-173X