Literature DB >> 3001115

Development of a sensitive protein A-gold immunoelectron microscopy method for detecting viral antigens in fluid specimens.

J F Hopley, F W Doane.   

Abstract

Protein A-colloidal gold immunoelectron microscopy (PAG IEM) has been employed to specifically detect rotavirus and enterovirus antigen in negatively stained fluid specimens. Unlike other IEM methods, PAG IEM can detect not only viral antigen associated with morphologically recognizable particles but also viral antigens of unrecognizable ultrastructure. This rapid and sensitive immunoassay was found to be applicable to virus-infected stool specimens as well as partially purified virus preparations. The sensitivity of viral antigen detection by PAG IEM was 2- to 40-fold greater than direct IEM and 200- to 1,000-fold greater than direct electron microscopy. In addition, PAG IEM appears to offer a more reliable and sensitive alternative to standard IEM for detection and quantitation of viral antibody.

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Year:  1985        PMID： 3001115     DOI： 10.1016/0166-0934(85)90014-x

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  9 in total

1.  Sensitive solid-phase immune electron microscopy double-antibody technique with gold-immunoglobulin G complexes for detecting rotavirus in cell culture and feces.

Authors:  B Wu; J B Mahony; G Simon; M A Chernesky
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

2.  Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy.

Authors:  T W Geisbert; J B Rhoderick; P B Jahrling
Journal:  J Clin Pathol       Date:  1991-06       Impact factor: 3.411

3.  Redirected infection of directly biotinylated recombinant adenovirus vectors through cell surface receptors and antigens.

Authors:  J S Smith; J R Keller; N C Lohrey; C S McCauslin; M Ortiz; K Cowan; S E Spence
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

4.  Immunohistochemical detection of porcine reproductive and respiratory syndrome virus using colloidal gold.

Authors:  R Magar; R Larochelle; Y Robinson; C Dubuc
Journal:  Can J Vet Res       Date:  1993-10       Impact factor: 1.310

Review 5.  Role of the virology laboratory in diagnosis and management of patients with central nervous system disease.

Authors:  T Chonmaitree; C D Baldwin; H L Lucia
Journal:  Clin Microbiol Rev       Date:  1989-01       Impact factor: 26.132

Review 6.  Electron microscopy for the rapid detection and identification of viruses from clinical specimens.

Authors:  C K Fong
Journal:  Yale J Biol Med       Date:  1989 Mar-Apr

7.  The use of convalescent sera in immune-electron microscopy to detect non-suspected/new viral agents.

Authors:  Antonio Lavazza; Cristiana Tittarelli; Monica Cerioli
Journal:  Viruses       Date:  2015-05-22       Impact factor: 5.048

8.  Immunoelectron Microscopy of Viral Antigens.

Authors:  Neetu M Gulati; Udana Torian; John R Gallagher; Audray K Harris
Journal:  Curr Protoc Microbiol       Date:  2019-06

9.  Development of protein A-gold immunoelectron microscopy for detection of bovine coronavirus in calves: comparison with ELISA and direct immunofluorescence of nasal epithelial cells.

Authors:  R A Heckert; L J Saif; G W Myers
Journal:  Vet Microbiol       Date:  1989-03       Impact factor: 3.293
  9 in total

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