Literature DB >> 3001114

In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections.

S A Gomes, J P Nascimento, M M Siqueira, M M Krawczuk, H G Pereira, W C Russell.   

Abstract

Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ hybridization with biotinylated probes. The technique was easy to perform, giving rapid results which were well correlated with those of immunofluorescence assays of the same samples. Adenoviruses of subgroups B, C and E were detected equally well by probes prepared either from purified adenovirus type 5 or from a plasmid (A1) carrying a cloned insertion of BamH1 fragments C and D of adenovirus type 2 in pAT 153. The use of stable non-radioactive probes makes in situ hybridization a feasible assay for use in clinical laboratories with moderate resources.

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Year:  1985        PMID： 3001114     DOI： 10.1016/0166-0934(85)90012-6

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  10 in total

1.  Epstein-Barr virus genomes in lymphoid cells: activation in mitosis and chromosomal location.

Authors:  C G Teo; B E Griffin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

2.  Evaluation of a commercial monoclonal antibody for detection of adenovirus antigen.

Authors:  M J August; A L Warford
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

3.  Direct detection and differentiation of fastidious and nonfastidious adenoviruses in stools by using a specific nonradioactive probe.

Authors:  C Niel; S A Gomes; J P Leite; H G Pereira
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

Review 4.  Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Brief review.

Authors:  M Norval; R W Bingham
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

Review 5.  Clinical laboratory applications of monoclonal antibodies.

Authors:  W J Payne; D L Marshall; R K Shockley; W J Martin
Journal:  Clin Microbiol Rev       Date:  1988-07       Impact factor: 26.132

6.  Nucleic acid hybridization for detection of cell culture-amplified adenovirus.

Authors:  C Huang; R Deibel
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

7.  Virus-associated RNAs (VA-I and VA-II). An efficient target for the detection of adenovirus infections by in situ hybridization.

Authors:  T C Wu; M D Kanayama; R H Hruban; W C Au; F B Askin; G M Hutchins
Journal:  Am J Pathol       Date:  1992-04       Impact factor: 4.307

8.  In vitro toxicity and efficacy of verdinexor, an exportin 1 inhibitor, on opportunistic viruses affecting immunocompromised individuals.

Authors:  Douglas G Widman; Savanna Gornisiewicz; Sharon Shacham; Sharon Tamir
Journal:  PLoS One       Date:  2018-10-17       Impact factor: 3.240

9.  Detection of rhinovirus RNA in nasal epithelial cells by in situ hybridization.

Authors:  C Bruce; P Chadwick; W al-Nakib
Journal:  J Virol Methods       Date:  1990-10       Impact factor: 2.014

10.  Biotinylated and radioactive cDNA probes in the detection by hybridization of bovine enteric coronavirus.

Authors:  A Verbeek; P Tijssen
Journal:  Mol Cell Probes       Date:  1988-09       Impact factor: 2.365
  10 in total

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