Literature DB >> 3001029

Purification and properties of shikimate kinase II from Escherichia coli K-12.

R C DeFeyter, J Pittard.   

Abstract

Shikimate kinase II was purified to near homogeneity from an Escherichia coli strain which overproduced the enzyme. The apparent Km of this isoenzyme for shikimate was 200 microM, and for ATP it was 160 microM. The Km for shikimate is approximately 100-fold lower than the Km of shikimate kinase I, suggesting that shikimate kinase II is the isoenzyme normally functioning in aromatic biosynthesis. Shikimate kinase II is dependent on metal ions for activity.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3001029      PMCID： PMC214414          DOI： 10.1128/jb.165.1.331-333.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  7 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  [The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].

Authors:  J MONOD; G COHEN-BAZIRE; M COHN
Journal:  Biochim Biophys Acta       Date:  1951-11

3.  Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.

Authors:  R C DeFeyter; J Pittard
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

4.  Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12.

Authors:  R C DeFeyter; B E Davidson; J Pittard
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Purification and characterization of shikimate kinase enzyme activity in Bacillus subtilis.

Authors:  L Huang; A L Montoya; E W Nester
Journal:  J Biol Chem       Date:  1975-10-10       Impact factor: 5.157

7.  Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12.

Authors:  B Ely; J Pittard
Journal:  J Bacteriol       Date:  1979-06       Impact factor: 3.490
  7 in total
  9 in total

1.  Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine.

Authors:  T Krell; J Maclean; D J Boam; A Cooper; M Resmini; K Brocklehurst; S M Kelly; N C Price; A J Lapthorn; J R Coggins
Journal:  Protein Sci       Date:  2001-06       Impact factor: 6.725

2.  Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli.

Authors:  A Løbner-Olesen; M G Marinus
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

3.  Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase.

Authors:  Wen-Chi Cheng; Yu-Ning Chang; Wen-Ching Wang
Journal:  J Bacteriol       Date:  2005-12       Impact factor: 3.490

4.  Biochemical, Kinetic, and Computational Structural Characterization of Shikimate Kinase from Methicillin-Resistant Staphylococcus aureus.

Authors:  Alejandro Favela-Candia; Alfredo Téllez-Valencia; Mara Campos-Almazán; Erick Sierra-Campos; Mónica Valdez-Solana; Jesús Oria-Hernández; Adriana Castillo-Villanueva; Hugo Nájera; Claudia Avitia-Domínguez
Journal:  Mol Biotechnol       Date:  2019-04       Impact factor: 2.695

5.  Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.

Authors:  R C DeFeyter; J Pittard
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

6.  Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12.

Authors:  R C DeFeyter; B E Davidson; J Pittard
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

7.  A reassessment of the relationship between aroK- and aroL-encoded shikimate kinase enzymes of Escherichia coli.

Authors:  M J Whipp; A J Pittard
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490

8.  Mecillinam resistance in Escherichia coli is conferred by loss of a second activity of the AroK protein.

Authors:  D Vinella; B Gagny; D Joseleau-Petit; R D'Ari; M Cashel
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

9.  Characterization of the TyrR Regulon in the Rhizobacterium Enterobacter ludwigii UW5 Reveals Overlap with the CpxR Envelope Stress Response.

Authors:  Thomas J D Coulson; René M Malenfant; Cheryl L Patten
Journal:  J Bacteriol       Date:  2020-12-07       Impact factor: 3.490
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.