BACKGROUND: Effects of pH (6-8), protein concentration (60-110, g kg-1 ), heating temperature (70-95 °C) and time (5-30 min) on physiochemical and rheological properties of thermal-induced polymerized whey protein isolate (PWP) were systematically investigated. Degree of denaturation, particle size, zeta potential, free sulfhydryl group content, surface hydrophobicity and apparent viscosity were determined. RESULTS: Heating whey protein above 75 °C at pH 7 or 8 resulted in denaturation of 80-90% whey protein. pH variation had a remarkable influence on particle size of samples (P < 0.05), whereas heating temperature and time did not generate significant changes. Zeta potential of PWP samples fell in the range of -30 to -40 mV. Free sulfhydryl group content of PWP samples decreased with increasing level regarding each factor. Surface hydrophobicity analysis showed that samples at higher pH or concentration became less hydrophobic, and increasing heating temperature or time resulted in higher hydrophobicity index. Time sweep test revealed that increasing protein concentration, heating temperature or time led to higher apparent viscosity. Flow behavior of PWP samples approached Newtonian character as protein concentration, heating temperature or time decreased. CONCLUSION: Systematic data may provide helpful information in designing a heating process for dairy products and application of PWP in the food industry.
BACKGROUND: Effects of pH (6-8), protein concentration (60-110, g kg-1 ), heating temperature (70-95 °C) and time (5-30 min) on physiochemical and rheological properties of thermal-induced polymerized whey protein isolate (PWP) were systematically investigated. Degree of denaturation, particle size, zeta potential, free sulfhydryl group content, surface hydrophobicity and apparent viscosity were determined. RESULTS: Heating whey protein above 75 °C at pH 7 or 8 resulted in denaturation of 80-90% whey protein. pH variation had a remarkable influence on particle size of samples (P < 0.05), whereas heating temperature and time did not generate significant changes. Zeta potential of PWP samples fell in the range of -30 to -40 mV. Free sulfhydryl group content of PWP samples decreased with increasing level regarding each factor. Surface hydrophobicity analysis showed that samples at higher pH or concentration became less hydrophobic, and increasing heating temperature or time resulted in higher hydrophobicity index. Time sweep test revealed that increasing protein concentration, heating temperature or time led to higher apparent viscosity. Flow behavior of PWP samples approached Newtonian character as protein concentration, heating temperature or time decreased. CONCLUSION: Systematic data may provide helpful information in designing a heating process for dairy products and application of PWP in the food industry.