Literature DB >> 30008983

Meningeal whole mount preparation and characterization of neural cells by flow cytometry.

Antoine Louveau1,2, Anthony J Filiano1,2, Jonathan Kipnis1,2.   

Abstract

Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable to diffuse into the brain parenchyma and influence the brain cells within the parenchyma, including neurons. In this unit, we will describe two protocols: analysis the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons).

Entities:  

Keywords:  Neuroimmunology; immune cells; meninges; neurons

Mesh:

Year:  2018        PMID: 30008983      PMCID: PMC6040815          DOI: 10.1002/cpim.50

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN: 1934-3671


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10.  Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts.

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