Yang Tan1, Qionghui Cheng1, Hang Yang1, Hongdong Li2, Nanpeng Gong1, Ding Liu3, Jinjin Wu1, Xia Lei4. 1. Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing 400042, China. 2. Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China. 3. Department of Infection Control, Daping Hospital, The Army Medical University, Chongqing 400042, China. 4. Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing 400042, China. Electronic address: leixia1979@sina.com.
Abstract
AIM: To investigate the effects of ALA-PDT on biofilm structure, virulence factor secretion, and quorum sensing (QS) in Pseudomonas aeruginosa. MATERIALS AND METHODS: We used confocal laser scanning microscopy (CLSM), an XTT assay, scanning electron microscopy (SEM), a virulence factor assay and qRT-PCR in this study. RESULTS: The XTT assay showed that ALA-PDT significantly inhibited the growth of planktonic P. aeruginosa. CLSM and SEM showed that ALA-PDT destroyed both bacterial and biofilm structures. The virulence factor assay showed that pyocyanin and elastase secretion were significantly inhibited in the ALA-PDT groups. qRT-PCR assays demonstrated that ALA-PDT significantly reduced the mRNA expression of QS-related genes. CONCLUSION: ALA-PDT kills planktonic and viable biofilm-associated P. aeruginosa cells, destroys biofilm structures, reduces virulence factor secretion and affects QS system gene expression.
AIM: To investigate the effects of ALA-PDT on biofilm structure, virulence factor secretion, and quorum sensing (QS) in Pseudomonas aeruginosa. MATERIALS AND METHODS: We used confocal laser scanning microscopy (CLSM), an XTT assay, scanning electron microscopy (SEM), a virulence factor assay and qRT-PCR in this study. RESULTS: The XTT assay showed that ALA-PDT significantly inhibited the growth of planktonic P. aeruginosa. CLSM and SEM showed that ALA-PDT destroyed both bacterial and biofilm structures. The virulence factor assay showed that pyocyanin and elastase secretion were significantly inhibited in the ALA-PDT groups. qRT-PCR assays demonstrated that ALA-PDT significantly reduced the mRNA expression of QS-related genes. CONCLUSION:ALA-PDT kills planktonic and viable biofilm-associated P. aeruginosa cells, destroys biofilm structures, reduces virulence factor secretion and affects QS system gene expression.
Authors: Tao Yang; Yang Tan; Wentao Zhang; Weijiang Yang; Jiefu Luo; Ling Chen; Hong Liu; Guihong Yang; Xia Lei Journal: Front Cell Dev Biol Date: 2020-12-04