Maryam Mahdavi Siuki1, Nafiseh Fazel Nasab2, Elham Barati3, Maryam Nezafat Firizi4, Tahereh Jalilvand5, Hasan Namdar Ahmadabad4. 1. a Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology , Tehran , Iran. 2. b Department of English Language , Eghbal Lahoori Institute of Higher Education , Mashhad , Iran. 3. c Department of Biology , Payame Noor University of Mashhad , Mashhad , Iran. 4. d Department of Pathobiology and Laboratory Sciences , North Khorasan University of Medical Sciences , Bojnurd , Iran. 5. e Department of Molecular Science, School of Medicine , North Khorasan University of Medical Sciences , Bojnurd , Iran.
Abstract
OBJECTIVE: Berberine is an isoquinoline derivative alkaloid with anti-inflammatory activity. In this study, we investigated the protective effects of berberine in prevention of LPS-induced abortion. MATERIALS AND METHODS: On the gestation day (GD) 9.5, the pregnant mice were injected with low, medium, and high doses of berberine or with PBS. After 4 h, berberine or PBS-pretreated mice were injected with LPS. On GD 11.5, blood samples and uterine tissues were collected from treated mice and percentage of abortion and serum levels of NO, TNF-α, IL-10, and IL12p70 were measured by macroscopic examination and sandwich ELISA, respectively. RESULTS: Our findings show that mice injected with berberine were resistant to LPS-induced abortion. We also found that this treatment prevents the reduction of IL-10 and the enhancement of NO, TNF-α, and IL-12p70 in LPS-treated pregnant mice. CONCLUSIONS: Taken together, our results suggest that berberine as an anti-inflammatory agent has protective effects on LPS-induced abortion by modulation of inflammatory/immune responses.
OBJECTIVE:Berberine is an isoquinoline derivative alkaloid with anti-inflammatory activity. In this study, we investigated the protective effects of berberine in prevention of LPS-induced abortion. MATERIALS AND METHODS: On the gestation day (GD) 9.5, the pregnant mice were injected with low, medium, and high doses of berberine or with PBS. After 4 h, berberine or PBS-pretreated mice were injected with LPS. On GD 11.5, blood samples and uterine tissues were collected from treated mice and percentage of abortion and serum levels of NO, TNF-α, IL-10, and IL12p70 were measured by macroscopic examination and sandwich ELISA, respectively. RESULTS: Our findings show that mice injected with berberine were resistant to LPS-induced abortion. We also found that this treatment prevents the reduction of IL-10 and the enhancement of NO, TNF-α, and IL-12p70 in LPS-treated pregnant mice. CONCLUSIONS: Taken together, our results suggest that berberine as an anti-inflammatory agent has protective effects on LPS-induced abortion by modulation of inflammatory/immune responses.
Authors: Giulio Petronio Petronio; Marco Alfio Cutuli; Irene Magnifico; Noemi Venditti; Laura Pietrangelo; Franca Vergalito; Antonella Pane; Giovanni Scapagnini; Roberto Di Marco Journal: Molecules Date: 2020-10-29 Impact factor: 4.411
Authors: Christiaan M Suttorp; René E M van Rheden; Natasja W M van Dijk; Maria P A C Helmich; Anne Marie Kuijpers-Jagtman; Frank A D T G Wagener Journal: Int J Mol Sci Date: 2020-07-29 Impact factor: 5.923