Literature DB >> 30003653

Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase.

Urikhan Sanzhaeva1,2, Xuan Xu1,3, Priyaankadevi Guggilapu1, Mark Tseytlin1,2, Valery V Khramtsov1,2, Benoit Driesschaert1,2,4.   

Abstract

Enzyme activities are well established biomarkers of many pathologies. Imaging enzyme activity directly in vivo may help to gain insight into the pathogenesis of various diseases but remains extremely challenging. In this communication, we report the use of EPR imaging (EPRI) in combination with a specially designed paramagnetic enzymatic substrate to map alkaline phosphatase activity with a high selectivity, thereby demonstrating the potential of EPRI to map enzyme activity.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  EPR spectroscopy; enzymes; imaging; nitroxide; phosphatase

Mesh：

Substances：

Year:  2018        PMID： 30003653      PMCID： PMC6327950          DOI： 10.1002/anie.201806851

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  26 in total

1.  Immobilized enzyme electron spin resonance: a method for detecting enzymatically generated transient radicals.

Authors:  Bradley E Sturgeon; Yeong-Renn Chen; Ronald P Mason
Journal:  Anal Chem       Date:  2003-10-01       Impact factor: 6.986

Review 2.  In Vivo Application of Proton-Electron Double-Resonance Imaging.

Authors:  Shun Kishimoto; Murali C Krishna; Valery V Khramtsov; Hideo Utsumi; David J Lurie
Journal:  Antioxid Redox Signal       Date:  2017-11-13       Impact factor: 8.401

3.  Reaction-Based Off-On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice.

Authors:  Yi Tan; Ling Zhang; Ka Ho Man; Raoul Peltier; Ganchao Chen; Huatang Zhang; Liyi Zhou; Feng Wang; Derek Ho; Shao Q Yao; Yi Hu; Hongyan Sun
Journal:  ACS Appl Mater Interfaces       Date:  2017-02-15       Impact factor: 9.229

4.  Enzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging.

Authors:  Gérard Audran; Lionel Bosco; Paul Brémond; Jean-Michel Franconi; Neha Koonjoo; Sylvain R A Marque; Philippe Massot; Philippe Mellet; Elodie Parzy; Eric Thiaudière
Journal:  Angew Chem Int Ed Engl       Date:  2015-09-17       Impact factor: 15.336

5.  Paramagnetic bradykinin analogues as substrates for angiotensin I-converting enzyme: Pharmacological and conformation studies.

Authors:  Luis Gustavo Deus Teixeira; Luciana Malavolta; Patrícia Alessandra Bersanetti; Shirley Schreier; Adriana K Carmona; Clovis R Nakaie
Journal:  Bioorg Chem       Date:  2016-10-27       Impact factor: 5.275

6.  Facile and Sensitive Near-Infrared Fluorescence Probe for the Detection of Endogenous Alkaline Phosphatase Activity In Vivo.

Authors:  Song-Jiao Li; Chun-Yan Li; Yong-Fei Li; Junjie Fei; Ping Wu; Bin Yang; Juan Ou-Yang; Shi-Xin Nie
Journal:  Anal Chem       Date:  2017-05-30       Impact factor: 6.986

7.  Selective On/Off-Nitroxides as Radical Probes to Investigate Non-radical Enzymatic Activity by Electron Paramagnetic Resonance.

Authors:  Indranil Duttagupta; Natacha Jugniot; Gérard Audran; Jean-Michel Franconi; Sylvain R A Marque; Philippe Massot; Philippe Mellet; Elodie Parzy; Eric Thiaudière; Nicolas Vanthuyne
Journal:  Chemistry       Date:  2018-05-03       Impact factor: 5.236

8.  Analogues containing the paramagnetic amino acid TOAC as substrates for angiotensin I-converting enzyme.

Authors:  Luis Gustavo de Deus Teixeira; Patrícia Alessandra Bersanetti; Shirley Schreier; Adriana Karaoglanovich Carmona; Clovis Ryuichi Nakaie
Journal:  FEBS Lett       Date:  2007-04-30       Impact factor: 4.124

9.  Conformational Properties of Seven Toac-Labeled Angiotensin I Analogues Correlate with Their Muscle Contraction Activity and Their Ability to Act as ACE Substrates.

Authors:  Luis Gustavo D Teixeira; Luciana Malavolta; Patrícia A Bersanetti; Shirley Schreier; Adriana K Carmona; Clovis R Nakaie
Journal:  PLoS One       Date:  2015-08-28       Impact factor: 3.240

10.  Overhauser-enhanced MRI of elastase activity from in vitro human neutrophil degranulation.

Authors:  Elodie Parzy; Véronique Bouchaud; Philippe Massot; Pierre Voisin; Neha Koonjoo; Damien Moncelet; Jean-Michel Franconi; Eric Thiaudière; Philippe Mellet
Journal:  PLoS One       Date:  2013-02-28       Impact factor: 3.240
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  5 in total

1.  Modular imaging system: Rapid scan EPR at 800 MHz.

Authors:  Oxana Tseytlin; Priyaankadevi Guggilapu; Andrey A Bobko; Hussien AlAhmad; Xuan Xu; Boris Epel; Ryan O'Connell; Emily H Hoblitzell; Timothy D Eubank; Valery V Khramtsov; Benoit Driesschaert; Eiad Kazkaz; Mark Tseytlin
Journal:  J Magn Reson       Date:  2019-06-08       Impact factor: 2.229

2.  Rapid Scan EPR imaging as a Tool for Magnetic Field Mapping.

Authors:  Oxana Tseytlin; Andrey A Bobko; Mark Tseytlin
Journal:  Appl Magn Reson       Date:  2020-09-25       Impact factor: 0.831

3.  Monitoring protein conformational changes using fluorescent nanoantennas.

Authors:  Scott G Harroun; Dominic Lauzon; Maximilian C C J C Ebert; Arnaud Desrosiers; Xiaomeng Wang; Alexis Vallée-Bélisle
Journal:  Nat Methods       Date:  2021-12-30       Impact factor: 28.547

Review 4.  EPR Everywhere.

Authors:  Joshua R Biller; Joseph E McPeak
Journal:  Appl Magn Reson       Date:  2021-01-24       Impact factor: 0.831

5.  Highly Sensitive "Off/On" EPR Probes to Monitor Enzymatic Activity.

Authors:  Sabrina Elkhanoufi; Rachele Stefania; Diego Alberti; Simona Baroni; Silvio Aime; Simonetta Geninatti Crich
Journal:  Chemistry       Date:  2022-03-03       Impact factor: 5.020
  5 in total

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