Jefferson da Rocha Tenório1, Leorik Pereira da Silva2, Marília Gabriela de Aguiar Xavier3, Thalita Santana4, George João Ferreira do Nascimento5, Ana Paula Veras Sobral3. 1. Department of Oral Pathology and Special Care Patients, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227-Butantã, São Paulo, SP, Zip code: 05508-900, Brazil. jeffersonrtenorio@usp.br. 2. Oral Pathology, Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte-UFRN, Natal, Rio Grande Do Norte, Brazil. 3. Oral Pathology, School of Dentistry, University of Pernambuco, Camaragibe, PE, Brazil. 4. Department of Oral Pathology and Special Care Patients, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227-Butantã, São Paulo, SP, Zip code: 05508-900, Brazil. 5. Biological Sciences Academic Center, School of Dentistry, Federal University of Campina Grande-UFCG, Patos, Paraíba, Brazil.
Abstract
PURPOSE: Salivary gland tumors are complex and have a great histomorphological diversity; more than 30 histological subtypes are currently described and the study of proteins that help understand and differentiate these tumors is essential. We aimed to analyze the immunoexpression of cyclooxygenase-2 (COX-2) and cyclin D1 proteins in pleomorphic adenomas (PA), mucoepidermoid carcinomas (MEC) and adenoid cystic carcinomas (AdCC) of salivary glands. METHODS: A total of 38 PA, 12 AdCC and 12 MEC underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed semi-quantitatively. For statistical analysis, a significance level was set at p ≤ 0.05. RESULTS: Overall, these tumors were more prevalent in women (n = 37). The mean age of these patients was 58-year-old and the parotid gland was the most affected anatomic site (n = 33). All cases of AdCC and MEC showed immunopositivity to cyclin D1; however, 39.5% of the PAs were negative (p < 0.001). Regarding COX-2 immunoexpression, we observed that all cases of CME were positive, whereas 60.5% of the PA and 75% of the CAC analyzed were completely negative (p = 0.042). CONCLUSIONS: The overexpression of COX-2, observed only in MEC, emphasizes that salivary gland tumors have different profiles. Cyclin D1 is more immunoexpressed in malignant tumors. Together, these immunohistochemical findings may be useful in differentiating the studied tumors.
PURPOSE:Salivary gland tumors are complex and have a great histomorphological diversity; more than 30 histological subtypes are currently described and the study of proteins that help understand and differentiate these tumors is essential. We aimed to analyze the immunoexpression of cyclooxygenase-2 (COX-2) and cyclin D1 proteins in pleomorphic adenomas (PA), mucoepidermoid carcinomas (MEC) and adenoid cystic carcinomas (AdCC) of salivary glands. METHODS: A total of 38 PA, 12 AdCC and 12 MEC underwent immunohistochemical study by the polymeric biotin-free technique. Immunopositive cells were analyzed semi-quantitatively. For statistical analysis, a significance level was set at p ≤ 0.05. RESULTS: Overall, these tumors were more prevalent in women (n = 37). The mean age of these patients was 58-year-old and the parotid gland was the most affected anatomic site (n = 33). All cases of AdCC and MEC showed immunopositivity to cyclin D1; however, 39.5% of the PAs were negative (p < 0.001). Regarding COX-2 immunoexpression, we observed that all cases of CME were positive, whereas 60.5% of the PA and 75% of the CAC analyzed were completely negative (p = 0.042). CONCLUSIONS: The overexpression of COX-2, observed only in MEC, emphasizes that salivary gland tumors have different profiles. Cyclin D1 is more immunoexpressed in malignant tumors. Together, these immunohistochemical findings may be useful in differentiating the studied tumors.
Authors: Seoung Wan Chae; Jin Hee Sohn; Dong-Hoon Kim; Yoon Jung Choi; Yong Lai Park; Kyungeun Kim; Young Hye Cho; Jung-Soo Pyo; Jun Ho Kim Journal: Yonsei Med J Date: 2011-05 Impact factor: 2.759
Authors: B Liu; J K Wen; B H Li; X M Fang; J J Wang; Y P Zhang; C J Shi; D Q Zhang; M Han Journal: Cell Death Dis Date: 2011-07-28 Impact factor: 8.469
Authors: Ji Won Lee; Jeong Hwan Park; Ja Hee Suh; Kyung Han Nam; Ji-Young Choe; Hae Yoen Jung; Ji Yoen Chae; Kyung Chul Moon Journal: Korean J Pathol Date: 2012-06-22