Literature DB >> 3000219

Enzymatic synthesis of [beta-32P]ADP using adenylate kinase and [gamma-32P]ATP.

J M Dawson, N D Cook, T J Peters.   

Abstract

An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretical obtainable.

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Year:  1985        PMID: 3000219     DOI: 10.1016/0003-2697(85)90600-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  3 in total

1.  Use of helper enzymes for ADP removal in infrared spectroscopic experiments: application to Ca2+-ATPase.

Authors:  Man Liu; Eeva-Liisa Karjalainen; Andreas Barth
Journal:  Biophys J       Date:  2005-02-24       Impact factor: 4.033

2.  Purification and properties of a novel nucleotide-hydrolysing enzyme (5'-nucleotidase) from Boophilus microplus.

Authors:  P Willadsen; J M Nielsen; G A Riding
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

3.  A simple, single-tube radioisotopic assay for the phosphorylation/inactivation activity of the pyruvate,orthophosphate dikinase regulatory protein.

Authors:  C M Smith; G Sarath; R Chollet
Journal:  Photosynth Res       Date:  1994-06       Impact factor: 3.573

  3 in total

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