Yasuhisa Munakata1, Manami Ueda1, Ryoka Kawahara-Miki2, Kazuki Kansaku1, Nobuhiko Itami1, Koumei Shirasuna1, Takehito Kuwayama1, Hisataka Iwata3. 1. Department of Animal Reproduction, Tokyo University of Agriculture, Funako 1737, Atsugi, Kanagawa, 243-0034, Japan. 2. NODAI Genome Research Center, Tokyo University of Agriculture, Setagaya, Tokyo, 156-8502, Japan. 3. Department of Animal Reproduction, Tokyo University of Agriculture, Funako 1737, Atsugi, Kanagawa, 243-0034, Japan. h1iwata@nodai.ac.jp.
Abstract
PURPOSE: Granulosa cell (GC) number in follicles is a simple characteristic of follicles. The present study examined the hypothesis that follicular fluid (FF) determines GC number and oocyte developmental ability and revealed the molecular background determining the number of follicular GCs. METHODS: FF was collected from antral follicles (3-5 mm in diameter), after which the number of GCs per follicle was determined for each donor gilt using real time PCR targeting single copy gene. GCs were analyzed by next-generation RNA sequencing and IPA pathway analysis. RESULTS: When oocyte cumulus cell-oocyte-complexes (COCs) were cultured in maturation medium containing 10% of each individual FF, the rate of development to the blastocyst stage was significantly correlated with the number of GCs in the donor gilt. In addition, when GCs were cultured in medium containing FF, the proliferative activity of the GCs was also significantly correlated to the number of GCs in the donor gilt. Moreover, when the FFs were categorized based on the number of GCs in the follicle, it was found that supplementation of culture media with GC-rich FF improved the developmental ability of oocytes compared to those supplemented with GC-poor FF. RNA sequencing and a pathway analysis of GCs from GC-rich and -poor follicles revealed the key regulatory pathway determining GC number in follicles. CONCLUSION: GC number may be a useful marker for "good" follicles and oocytes, and the characteristics of the FFs determine granulosa cell number and oocyte developmental ability.
PURPOSE: Granulosa cell (GC) number in follicles is a simple characteristic of follicles. The present study examined the hypothesis that follicular fluid (FF) determines GC number and oocyte developmental ability and revealed the molecular background determining the number of follicular GCs. METHODS: FF was collected from antral follicles (3-5 mm in diameter), after which the number of GCs per follicle was determined for each donor gilt using real time PCR targeting single copy gene. GCs were analyzed by next-generation RNA sequencing and IPA pathway analysis. RESULTS: When oocyte cumulus cell-oocyte-complexes (COCs) were cultured in maturation medium containing 10% of each individual FF, the rate of development to the blastocyst stage was significantly correlated with the number of GCs in the donor gilt. In addition, when GCs were cultured in medium containing FF, the proliferative activity of the GCs was also significantly correlated to the number of GCs in the donor gilt. Moreover, when the FFs were categorized based on the number of GCs in the follicle, it was found that supplementation of culture media with GC-rich FF improved the developmental ability of oocytes compared to those supplemented with GC-poor FF. RNA sequencing and a pathway analysis of GCs from GC-rich and -poor follicles revealed the key regulatory pathway determining GC number in follicles. CONCLUSION: GC number may be a useful marker for "good" follicles and oocytes, and the characteristics of the FFs determine granulosa cell number and oocyte developmental ability.
Authors: Tatiana D Saccon; Monique T Rovani; Driele N Garcia; Jorgea Pradiee; Rafael G Mondadori; Luis Augusto X Cruz; Carlos C Barros; Yimin Fang; Samuel McFadden; Jeffrey B Mason; Andrzej Bartke; Michal M Masternak; Augusto Schneider Journal: Geroscience Date: 2021-05-05 Impact factor: 7.581