Rebeca Pérez-Morales1, Alberto González-Zamora2, María Fernanda González-Delgado1, Esperanza Y Calleros Rincón1, Edgar H Olivas Calderón1, Ollin Celeste Martínez-Ramírez3, Julieta Rubio4. 1. Laboratorio de Biología Celulary Molecular, Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Durango, México. 2. Laboratorio de Biología Evolutiva. Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Durango, México. 3. Facultad de Nutrición, Universidad Autónoma del Estado de Morelos, Morelos, México. 4. Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, México.
Abstract
BACKGROUND AND PURPOSE: Smoking is a major public health problem worldwide. Polymorphisms in CHRNA3, CHRNA5, and CHRNB4 receptors play a critical role in nicotine dependence, lung cancer (LC) risk, and chronic obstructive pulmonary disease (COPD). This study characterized the CHRNA3 rs1051730 and CHRNA5 rs16969968 polymorphisms in a Mexican population and its association with nicotine dependence, LC, and COPD. METHODS: The study included 312 healthy individuals, 74 LC cases and 117 COPD cases. Genotyping was performed using TaqMan probes, and the data were analyzed using logistic regression adjusted for covariates. RESULTS: The polymorphism CHRNA3 rs1051730 and CHRNA5 rs16969968 were in the Hardy-Weinberg equilibrium and the allelic frequency of the A allele was 0.15, for both polymorphisms. The smokers were stratified in heavy smokers and moderate/light smokers, and we found in A alleles an OR = 2.86 (P = 0.01) to CHRNA3 rs1051730 and OR = 3.12 (P = 0.03) to CHRNA5 rs16969968. In addition, the A alleles in CHRNA3 rs1051730 and CHRNA5 rs16969968 were associated with the risk for LC (OR = 1.66, P = 0.07 and OR = 1.57, P = 0.1, respectively) and for COPD (OR = 2.04, P = 0.01 and OR = 1.91, P = 0.02, respectively). CONCLUSION: CHRNA3/5 polymorphisms are associated with nicotine dependence, LC, and COPD in Mexicans.
BACKGROUND AND PURPOSE: Smoking is a major public health problem worldwide. Polymorphisms in CHRNA3, CHRNA5, and CHRNB4 receptors play a critical role in nicotine dependence, lung cancer (LC) risk, and chronic obstructive pulmonary disease (COPD). This study characterized the CHRNA3rs1051730 and CHRNA5rs16969968 polymorphisms in a Mexican population and its association with nicotine dependence, LC, and COPD. METHODS: The study included 312 healthy individuals, 74 LC cases and 117 COPD cases. Genotyping was performed using TaqMan probes, and the data were analyzed using logistic regression adjusted for covariates. RESULTS: The polymorphism CHRNA3rs1051730 and CHRNA5rs16969968 were in the Hardy-Weinberg equilibrium and the allelic frequency of the A allele was 0.15, for both polymorphisms. The smokers were stratified in heavy smokers and moderate/light smokers, and we found in A alleles an OR = 2.86 (P = 0.01) to CHRNA3rs1051730 and OR = 3.12 (P = 0.03) to CHRNA5rs16969968. In addition, the A alleles in CHRNA3rs1051730 and CHRNA5rs16969968 were associated with the risk for LC (OR = 1.66, P = 0.07 and OR = 1.57, P = 0.1, respectively) and for COPD (OR = 2.04, P = 0.01 and OR = 1.91, P = 0.02, respectively). CONCLUSION:CHRNA3/5 polymorphisms are associated with nicotine dependence, LC, and COPD in Mexicans.
Authors: Krzysztof Chmielowiec; Jolanta Chmielowiec; Aleksandra Strońska-Pluta; Grzegorz Trybek; Małgorzata Śmiarowska; Aleksandra Suchanecka; Grzegorz Woźniak; Aleksandra Jaroń; Anna Grzywacz Journal: Int J Environ Res Public Health Date: 2022-08-23 Impact factor: 4.614