Literature DB >> 29992457

Affinity determination of biomolecules: a kinetic model for the analysis of pre-equilibrium titration curves.

Emilie Vanhove1, Marc Vanhove2,3.   

Abstract

Equilibrium titration curves have been used to determine the affinity of biomolecules using a variety of platforms and technologies. Such measurements, however, are experimentally limited by the time that is needed for the system to reach the equilibrium, which may exceed the time during which the studied biomolecules are stable. We propose here a kinetic model to analyze titration curves obtained prior to full equilibration of the system.

Keywords:  Affinity measurements; High-affinity interactions; Pre-equilibrium titration curves; Therapeutic antibodies

Mesh：

Year:  2018        PMID： 29992457     DOI： 10.1007/s00249-018-1318-y

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  9 in total

1.  Designed ankyrin repeat proteins (DARPins) from research to therapy.

Authors:  Rastislav Tamaskovic; Manuel Simon; Nikolas Stefan; Martin Schwill; Andreas Plückthun
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

2.  Rapid affinity measurement of protein-protein interactions in a microfluidic platform.

Authors:  Hossein Salimi-Moosavi; Palaniswami Rathanaswami; Surendran Rajendran; Mike Toupikov; John Hill
Journal:  Anal Biochem       Date:  2012-04-26       Impact factor: 3.365

3.  Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration.

Authors:  Cornelia Haenel; Marion Satzger; Daniela Della Ducata; Ralf Ostendorp; Bodo Brocks
Journal:  Anal Biochem       Date:  2005-04-01       Impact factor: 3.365

4.  Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics.

Authors:  L Nieba; A Krebber; A Plückthun
Journal:  Anal Biochem       Date:  1996-02-15       Impact factor: 3.365

5.  Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assay.

Authors:  F Hardy; L Djavadi-Ohaniance; M E Goldberg
Journal:  J Immunol Methods       Date:  1997-01-15       Impact factor: 2.303

6.  Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors.

Authors:  R Karlsson; A Fält
Journal:  J Immunol Methods       Date:  1997-01-15       Impact factor: 2.303

7.  Solution Equilibrium Titration for High-Throughput Affinity Estimation of Unpurified Antibodies and Antibody Fragments.

Authors:  Daniela Della Ducata; Jan Jaehrling; Cornelia Hänel; Marion Satzger; Meike Wolber; Ralf Ostendorp; Stefan Pabst; Bodo Brocks
Journal:  J Biomol Screen       Date:  2015-07-15

8.  Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors.

Authors:  R Karlsson
Journal:  Anal Biochem       Date:  1994-08-15       Impact factor: 3.365

9.  High throughput solution-based measurement of antibody-antigen affinity and epitope binning.

Authors:  Patricia Estep; Felicia Reid; Claire Nauman; Yuqi Liu; Tingwan Sun; Joanne Sun; Yingda Xu
Journal:  MAbs       Date:  2013 Mar-Apr       Impact factor: 5.857
  9 in total
  2 in total

1.  KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements.

Authors:  Diana Spiegelberg; Jonas Stenberg; Pascale Richalet; Marc Vanhove
Journal:  Eur Biophys J       Date:  2021-07-24       Impact factor: 1.733

2.  A novel assay based on pre-equilibrium titration curves for the determination of enzyme inhibitor binding kinetics.

Authors:  Bernard Noppen; Anouk Vanbelle; Alan W Stitt; Marc Vanhove
Journal:  Eur Biophys J       Date:  2021-06-22       Impact factor: 1.733
  2 in total

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