Literature DB >> 2998947

Use of the deoxyinosine-containing probe to isolate and sequence cDNA encoding the fusion (F) glycoprotein of Sendai virus (HVJ).

N Miura, E Ohtsuka, N Yamaberi, M Ikehara, T Uchida, Y Okada.   

Abstract

A synthetic 20-mer based on the known amino acid (aa) sequence of the N-terminus of Sendai virus F1 polypeptide was synthesized. Using this dI-probe, which contained deoxyinosines at all six ambiguous codon positions, we isolated clones carrying cDNAs for the F mRNA of Sendai virus. Nucleotide (nt) sequence analysis revealed a long open reading frame (ORF) that encodes a protein of 565 aa. Thus, this type of dI-probes should prove useful for selecting cDNA clones, when the aa sequence is known and is characterized by high codon redundancy.

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Year:  1985        PMID： 2998947     DOI： 10.1016/0378-1119(85)90228-8

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  3 in total

1.  Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii.

Authors:  S Booker; J Stubbe
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-15       Impact factor: 11.205

2.  Nearest-neighbor thermodynamics of deoxyinosine pairs in DNA duplexes.

Authors:  Norman E Watkins; John SantaLucia
Journal:  Nucleic Acids Res       Date:  2005-11-01       Impact factor: 16.971

3.  A Unique Primer with an Inosine Chain at the 5'-Terminus Improves the Reliability of SNP Analysis Using the PCR-Amplified Product Length Polymorphism Method.

Authors:  Hideki Shojo; Mayumi Tanaka; Ryohei Takahashi; Tsuneo Kakuda; Noboru Adachi
Journal:  PLoS One       Date:  2015-09-18       Impact factor: 3.240
  3 in total

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