Katarzyna Gawron1, Anna Ochała-Kłos2, Zuzanna Nowakowska1, Grzegorz Bereta1, Katarzyna Łazarz-Bartyzel3, Aleksander M Grabiec1, Paweł Plakwicz4, Renata Górska4, Andrzej Fertala5, Maria Chomyszyn-Gajewska3, Jan Potempa1,6. 1. Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. 2. Department of Anatomy, Medical College, Jagiellonian University, Krakow, Poland. 3. Department of Periodontology and Oral Medicine, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland. 4. Department of Periodontology, Medical University of Warsaw, Warsaw, Poland. 5. Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. 6. Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, Kentucky, USA.
Abstract
OBJECTIVES: To investigate the processes associated with the excessive production of collagen I in hereditary gingival fibromatosis (HGF). MATERIALS AND METHODS: Three HGF subjects and five controls were enrolled in the study. Histomorphological and immunohistological analyses were performed on gingival tissues. The expression of heat-shock protein 47 (HSP47), collagen I, transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) by gingival fibroblasts isolated from HGF and controls was analysed using qRT-PCR, Western blotting and ELISA. RESULTS: Considerable accumulation of fibrotic fibrils and increased synthesis of HSP47 were noted in HGF gingival tissues. The synthesis of collagen I, HSP47, TGF-β1, CTGF and TIMP-1 was significantly elevated in HGF gingival fibroblasts compared with controls, while the production of MMP-1 was decreased. CONCLUSIONS: We report that fibrosis in HGF gingival tissues is associated with increased synthesis of HSP47. This finding was confirmed by an in vitro study, where excessive production of collagen I was associated with increased synthesis of HSP47, TGF-β1 and CTGF by HGF gingival fibroblasts. Moreover, the shift in the TIMP-1/MMP-1 ratio identifies increased synthesis of TIMP-1 as one of the processes associated with collagen I overproduction in HGF fibroblasts.
OBJECTIVES: To investigate the processes associated with the excessive production of collagen I in hereditary gingival fibromatosis (HGF). MATERIALS AND METHODS: Three HGF subjects and five controls were enrolled in the study. Histomorphological and immunohistological analyses were performed on gingival tissues. The expression of heat-shock protein 47 (HSP47), collagen I, transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) by gingival fibroblasts isolated from HGF and controls was analysed using qRT-PCR, Western blotting and ELISA. RESULTS: Considerable accumulation of fibrotic fibrils and increased synthesis of HSP47 were noted in HGF gingival tissues. The synthesis of collagen I, HSP47, TGF-β1, CTGF and TIMP-1 was significantly elevated in HGF gingival fibroblasts compared with controls, while the production of MMP-1 was decreased. CONCLUSIONS: We report that fibrosis in HGF gingival tissues is associated with increased synthesis of HSP47. This finding was confirmed by an in vitro study, where excessive production of collagen I was associated with increased synthesis of HSP47, TGF-β1 and CTGF by HGF gingival fibroblasts. Moreover, the shift in the TIMP-1/MMP-1 ratio identifies increased synthesis of TIMP-1 as one of the processes associated with collagen I overproduction in HGF fibroblasts.
Authors: Anna Maksylewicz; Agnieszka Bysiek; Katarzyna B Lagosz; Justyna M Macina; Malgorzata Kantorowicz; Grzegorz Bereta; Maja Sochalska; Katarzyna Gawron; Maria Chomyszyn-Gajewska; Jan Potempa; Aleksander M Grabiec Journal: Front Immunol Date: 2019-04-30 Impact factor: 7.561
Authors: Elisa Roztocil; Christine L Hammond; Mithra O Gonzalez; Steven E Feldon; Collynn F Woeller Journal: Sci Rep Date: 2020-05-21 Impact factor: 4.379
Authors: Karolina Strzelec; Agata Dziedzic; Katarzyna Łazarz-Bartyzel; Aleksander M Grabiec; Ewa Gutmajster; Tomasz Kaczmarzyk; Paweł Plakwicz; Katarzyna Gawron Journal: Orphanet J Rare Dis Date: 2021-11-24 Impact factor: 4.123