| Literature DB >> 29988096 |
Matthias Meurer1, Yuanqiang Duan1, Ehud Sass2, Ilia Kats1, Konrad Herbst1, Benjamin C Buchmuller1, Verena Dederer1, Florian Huber1,3, Daniel Kirrmaier4, Martin Štefl1,5, Koen Van Laer6, Tobias P Dick6, Marius K Lemberg1, Anton Khmelinskii7,8, Emmanuel D Levy9, Michael Knop10,11.
Abstract
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.Entities:
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Year: 2018 PMID： 29988096 DOI： 10.1038/s41592-018-0045-8
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547