Literature DB >> 29987781

Isolation and Characterization of Mouse Neutrophils.

Niki D J Ubags1, Benjamin T Suratt2.   

Abstract

Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.

Entities:  

Keywords:  Blood; Density gradient; Isolation; Lung; Marrow; Neutrophils

Mesh:

Substances:

Year:  2018        PMID: 29987781     DOI: 10.1007/978-1-4939-8570-8_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  4 in total

1.  Midkine promotes kidney injury in diabetic kidney disease by increasing neutrophil extracellular traps formation.

Authors:  Gaohong Liu; Xiaojun Ren; Yousong Li; Han Li
Journal:  Ann Transl Med       Date:  2022-06

2.  Natural NADH and FAD Autofluorescence as Label-Free Biomarkers for Discriminating Subtypes and Functional States of Immune Cells.

Authors:  Sarah Lemire; Oana-Maria Thoma; Lucas Kreiss; Simon Völkl; Oliver Friedrich; Markus F Neurath; Sebastian Schürmann; Maximilian J Waldner
Journal:  Int J Mol Sci       Date:  2022-02-20       Impact factor: 5.923

3.  Bone marrow is the preferred source for isolation of rat neutrophils and the subsequent acquisition of neutrophil extracellular traps.

Authors:  Li He; Xiaohui Pan; Xiaoxin Zhang; Guonian Zhu; Ruiqi Liu; Chaoyi Qin
Journal:  Ann Transl Med       Date:  2022-08

4.  GSDMD-dependent neutrophil extracellular traps promote macrophage-to-myofibroblast transition and renal fibrosis in obstructive nephropathy.

Authors:  Yujia Wang; Yinshuang Li; Zhimin Chen; Ying Yuan; Qinglin Su; Keng Ye; Caiming Chen; Guoping Li; Yankun Song; Hong Chen; Yanfang Xu
Journal:  Cell Death Dis       Date:  2022-08-08       Impact factor: 9.685

  4 in total

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