Literature DB >> 29986132

Thiol-Epoxy "Click" Chemistry to Engineer Cytocompatible PEG-Based Hydrogel for siRNA-Mediated Osteogenesis of hMSCs.

Cong Truc Huynh1, Fangze Liu1, Yuxuan Cheng1, Katherine A Coughlin2, Eben Alsberg1,3.   

Abstract

Thiol-epoxy "click" chemistry is employed for the first time to engineer a new cytocompatible PEG-based hydrogel system in aqueous media with the ability to encapsulate human mesenchymal stem cells (hMSCs) and control their fate for tissue regeneration. Cells were easily encapsulated into the hydrogels and exhibited high cell viability over 4 weeks of culture regardless of the presence of siRNA, complexed with polyethylenimine (PEI) in the form of siRNA/PEI nanocomplexes, indicating the biocompatibility of the developed hydrogel. Loading pro-osteogenic siNoggin in the hydrogel significantly enhanced the osteogenesis of encapsulated hMSCs, demonstrating the potential application of this system in tissue engineering.

Entities:  

Keywords:  RNAi; biomaterial; click chemistry; hydrogel; stem cell differentiation; tissue regeneration

Mesh：

Substances：

Year:  2018        PMID： 29986132      PMCID： PMC6930143          DOI： 10.1021/acsami.8b07167

Source DB:  PubMed          Journal:  ACS Appl Mater Interfaces        ISSN： 1944-8244            Impact factor:   9.229


  37 in total

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Authors:  Jeanie L Drury; David J Mooney
Journal:  Biomaterials       Date:  2003-11       Impact factor: 12.479

2.  EZH2 and KDM6A act as an epigenetic switch to regulate mesenchymal stem cell lineage specification.

Authors:  Sarah Hemming; Dimitrios Cakouros; Sandra Isenmann; Lachlan Cooper; Danijela Menicanin; Andrew Zannettino; Stan Gronthos
Journal:  Stem Cells       Date:  2014-03       Impact factor: 6.277

3.  Photocrosslinkable, biodegradable hydrogels with controlled cell adhesivity for prolonged siRNA delivery to hMSCs to enhance their osteogenic differentiation.

Authors:  Minh Khanh Nguyen; Alexandra McMillan; Cong Truc Huynh; Daniel S Schapira; Eben Alsberg
Journal:  J Mater Chem B       Date:  2016-12-14       Impact factor: 6.331

4.  Functionalized, biodegradable hydrogels for control over sustained and localized siRNA delivery to incorporated and surrounding cells.

Authors:  Khanh Nguyen; Phuong Ngoc Dang; Eben Alsberg
Journal:  Acta Biomater       Date:  2012-08-16       Impact factor: 8.947

5.  In Situ "Clickable" Zwitterionic Starch-Based Hydrogel for 3D Cell Encapsulation.

Authors:  Dianyu Dong; Junjie Li; Man Cui; Jinmei Wang; Yuhang Zhou; Liu Luo; Yufei Wei; Lei Ye; Hong Sun; Fanglian Yao
Journal:  ACS Appl Mater Interfaces       Date:  2016-02-09       Impact factor: 9.229

6.  In situ crosslinkable hyaluronan hydrogels for tissue engineering.

Authors:  Xiao Zheng Shu; Yanchun Liu; Fabio S Palumbo; Yi Luo; Glenn D Prestwich
Journal:  Biomaterials       Date:  2004 Mar-Apr       Impact factor: 12.479

7.  Collagen-mimetic peptide-modifiable hydrogels for articular cartilage regeneration.

Authors:  Paresh A Parmar; Lesley W Chow; Jean-Philippe St-Pierre; Christine-Maria Horejs; Yong Y Peng; Jerome A Werkmeister; John A M Ramshaw; Molly M Stevens
Journal:  Biomaterials       Date:  2015-04-11       Impact factor: 12.479

8.  Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency.

Authors:  Kien Nguyen; Biswajit Das; Curtis Dobrowolski; Jonathan Karn
Journal:  mBio       Date:  2017-02-28       Impact factor: 7.867

9.  Localized, targeted, and sustained siRNA delivery.

Authors:  Melissa D Krebs; Eben Alsberg
Journal:  Chemistry       Date:  2011-02-21       Impact factor: 5.236

10.  Sustained delivery of siRNA/PEI complex from in situ forming hydrogels potently inhibits the proliferation of gastric cancer.

Authors:  Hao Peng; Huawei Yang; Liwei Song; Zheng Zhou; Jinwen Sun; Yunfeng Du; Keyu Lu; Tao Li; Aiguo Yin; Jianhua Xu; Shidong Wei
Journal:  J Exp Clin Cancer Res       Date:  2016-03-31
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  9 in total

1.  Rational design of hydrogels to enhance osteogenic potential.

Authors:  Soyon Kim; Min Lee
Journal:  Chem Mater       Date:  2020-11-05       Impact factor: 9.811

2.  Synthesis and Characterization of Multifunctional Secondary Thiol Hardeners Using 3-Mercaptobutanoic Acid and Their Thiol-Epoxy Curing Behavior.

Authors:  Seung-Mo Hong; Seok-Ho Hwang
Journal:  ACS Omega       Date:  2022-06-10

3.  Hydrogel microspheres for spatiotemporally controlled delivery of RNA and silencing gene expression within scaffold-free tissue engineered constructs.

Authors:  Alexandra McMillan; Minh Khanh Nguyen; Cong Truc Huynh; Samantha M Sarett; Peilin Ge; Melanie Chetverikova; Kien Nguyen; David Grosh; Craig L Duvall; Eben Alsberg
Journal:  Acta Biomater       Date:  2021-01-16       Impact factor: 8.947

4.  Optimization of synthetic parameters of high-purity trifunctional mercaptoesters and their curing behavior for the thiol-epoxy click reaction.

Authors:  Seung-Mo Hong; Oh Young Kim; Seok-Ho Hwang
Journal:  RSC Adv       Date:  2021-10-22       Impact factor: 3.361

5.  Covalently tethering siRNA to hydrogels for localized, controlled release and gene silencing.

Authors:  Minh Khanh Nguyen; Cong Truc Huynh; Alex Gilewski; Samantha E Wilner; Keith E Maier; Nicholas Kwon; Mathew Levy; Eben Alsberg
Journal:  Sci Adv       Date:  2019-08-28       Impact factor: 14.136

Review 6.  The Delivery of RNA-Interference Therapies Based on Engineered Hydrogels for Bone Tissue Regeneration.

Authors:  Tingting Yu; Hufei Wang; Yunfan Zhang; Xing Wang; Bing Han
Journal:  Front Bioeng Biotechnol       Date:  2020-05-12

7.  Inflammatory Modulation of Polyethylene Glycol-AuNP for Regulation of the Neural Differentiation Capacity of Mesenchymal Stem Cells.

Authors:  Huey-Shan Hung; Wei-Chien Kao; Chiung-Chyi Shen; Kai-Bo Chang; Cheng-Ming Tang; Meng-Yin Yang; Yi-Chin Yang; Chun-An Yeh; Jia-Jhan Li; Hsien-Hsu Hsieh
Journal:  Cells       Date:  2021-10-22       Impact factor: 6.600

Review 8.  Self-Assemblable Polymer Smart-Blocks for Temperature-Induced Injectable Hydrogel in Biomedical Applications.

Authors:  Thai Thanh Hoang Thi; Le Hoang Sinh; Dai Phu Huynh; Dai Hai Nguyen; Cong Huynh
Journal:  Front Chem       Date:  2020-01-31       Impact factor: 5.221

9.  Facile Preparation of Mechanically Robust and Functional Silica/Cellulose Nanofiber Gels Reinforced with Soluble Polysaccharides.

Authors:  Marco Beaumont; Elisabeth Jahn; Andreas Mautner; Stefan Veigel; Stefan Böhmdorfer; Antje Potthast; Wolfgang Gindl-Altmutter; Thomas Rosenau
Journal:  Nanomaterials (Basel)       Date:  2022-03-08       Impact factor: 5.719
  9 in total

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