Gisselle Escobar1, Alejandro Escobar2, Gabriel Ascui1, Fabián I Tempio1,3, María C Ortiz2, Claudio A Pérez4,3, Mercedes N López1,3. 1. Disciplinary Program of Immunology, Faculty of Medicine, Institute of Biomedical Sciences, University of Chile, Independencia, Santiago 8380453, Chile. 2. Biological Science Program, Research Institute of Dental Sciences, Faculty of Dentistry, University of Chile, Independencia, Santiago 8380492, Chile. 3. Millenium Institute on Immunology & immunotherapy, University of Chile, Santiago 8330025, Chile. 4. Cell Therapy Laboratory, Blood Bank Service, University of Chile Clinical Hospital, Independencia, Santiago 8380456, Chile.
Abstract
AIM: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. MATERIALS & METHODS: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24 h stimulation with P-PRP or S-PRP. RESULTS: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker. However, these same macrophages presented different levels of CD163, CD86 as well as different IL-10 secretion capacities after 24 h incubation. CONCLUSION: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.
AIM: This study aimed to evaluate the effect of two platelet preparations used in the clinic, pure platelet-rich plasma (P-PRP) and the supernatant of calcium-activated P-PRP (S-PRP), on the phenotype of human macrophages. MATERIALS & METHODS: Surface markers and cytokine production of human monocyte-derived macrophages were analyzed after 24 h stimulation with P-PRP or S-PRP. RESULTS: P-PRP and S-PRP present no difference in the expression of CD206, a M2 tissue-repair macrophage-related marker. However, these same macrophages presented different levels of CD163, CD86 as well as different IL-10 secretion capacities after 24 h incubation. CONCLUSION: These platelet preparations do not have an equivalent biological effect over macrophages, which suggest that they may present different clinical regenerative potentials.
Authors: Ronaldo J F C do Amaral; Noora M A Zayed; Elena I Pascu; Brenton Cavanagh; Chris Hobbs; Francesco Santarella; Christopher R Simpson; Ciara M Murphy; Rukmani Sridharan; Arlyng González-Vázquez; Barry O'Sullivan; Fergal J O'Brien; Cathal J Kearney Journal: Front Bioeng Biotechnol Date: 2019-12-03