Gloria Pelizzo1, Maria Antonietta Avanzini2, Antonia Icaro Cornaglia3, Annalisa De Silvestri4, Melissa Mantelli2, Paola Travaglino2, Stefania Croce2, Piero Romano5, Luigi Avolio5, Giulio Iacob5, Massimo Dominici6, Valeria Calcaterra7. 1. Pediatric Surgery Unit, Children's Hospital, Istituto Mediterraneo di Eccellenza Pediatrica, Palermo, Italy. 2. Immunology & Transplantation Laboratory, Cell Factory, Pediatric Hematology Oncology Unit, Department of Maternal & Children's Health, Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy. 3. Department of Public Health, Experimental Medicine & Forensic, Histology & Embryology Unit, University of Pavia, Italy. 4. Biometry & Clinical Epidemiology, Scientific Direction, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. 5. Pediatric Surgery Unit, Department of Maternal & Children's Health, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. 6. Laboratory of Cellular Therapies Department of Medical & Surgical Sciences for Children & Adults University Hospital of Modena & Reggio Emilia, Italy. 7. Pediatric Unit, Department of Internal Medicine, University of Pavia & Department of Maternal & Children's Health, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Abstract
AIM: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model. METHODS: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections. Histological examination was performed postinoculation. RESULTS: EV-treated wounds exhibited a better restoration compared with the counterpart MSC treatment. ASC-EV-treated wounds were significantly better than BM-EVs (p = 0.036). CONCLUSION: EV topical inoculation provides restored architecture during cutaneous wound healing and represents a promising solution for regenerative medicine in children.
AIM: We evaluated the effects of the intradermal injection of extracellular vesicles (EVs) derived from adipose stem cells (ASC-EVs) and bone marrow cells (BM-EVs) in an experimental cutaneous wound repair model. METHODS: Mesenchymal stem cells (MSCs) were in vitro expanded from adipose (ASC) or BM tissues (BM-MSC) of rabbits. EVs were separated from the supernatants of confluent ASC and BM-MSCs. Two skin wounds were induced in each animal and treated with MSC or EV injections. Histological examination was performed postinoculation. RESULTS: EV-treated wounds exhibited a better restoration compared with the counterpart MSC treatment. ASC-EV-treated wounds were significantly better than BM-EVs (p = 0.036). CONCLUSION: EV topical inoculation provides restored architecture during cutaneous wound healing and represents a promising solution for regenerative medicine in children.
Authors: Evi M Morandi; Christian Ploner; Dolores Wolfram; Christoph Tasch; Lucie Dostal; Felix Ortner; Gerhard Pierer; Ralph Verstappen Journal: Int Wound J Date: 2019-10-13 Impact factor: 3.315
Authors: Bibi S Subhan; Michelle Ki; Alexandra Verzella; Shruthi Shankar; Piul S Rabbani Journal: Adv Wound Care (New Rochelle) Date: 2021-12-30 Impact factor: 4.947