Mehmet Evren Okur1, Şule Ayla2,3, Derya Çiçek Polat4, Mehmet Y Günal5, Ayşegül Yoltaş6, Özge Biçeroğlu2. 1. Faculty of Pharmacy, Department of Pharmacology, Anadolu University, Eskişehir, Turkey. 2. Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey. 3. Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Istanbul, Turkey. 4. Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara University, Ankara, Turkey. 5. Faculty of Medicine, Department of Physiology, Alanya Alaaddin Keykubat University, Antalya, Turkey. 6. Faculty of Science, Fundamental and Industrial Microbiology Division, Department of Biology, Ege University, Izmir, Turkey.
Abstract
OBJECTIVES: The objective of this study was to investigate the wound healing activity of Capparis ovata var. palaestina fruit extract in mice. For this aim, wound healing, in vitro antimicrobial and antioxidant activities, HPLC, phenolic and flavonoid compounds analyses were performed. METHODS: The wound healing effect was tested by excisional wound model. Wound closure was measured for 14 days and at 14th day wound healing was assessed by levels of TGF-β, VEGF, COL1A1 and angiogenesis, granulation tissue thickness, epidermal and dermal regeneration. The antioxidant activity was calculated by DPPH and ABTS free radical scavenging assays. Antimicrobial ability was evaluated by minimum inhibitory concentration and agar well diffusion tests. KEY FINDINGS: The extract indicated significant antioxidant activity while it also exhibited antimicrobial activity. Rutin was found in the extract according to HPLC study. Moreover, the extract was found to have rich phenolic and flavonoid contents. Histological evaluation showed that extract group induced significant (P < 0.001) wound healing activity compared to control group. Furthermore, extract group increased wound healing rates by promoting granulation tissue, epidermal regeneration, angiogenesis, collagen, TGF-β and VEGF. CONCLUSIONS: The results clarified that the extract possesses antioxidant, antimicrobial activity and thus could provide a valuable contribution to the wound healing.
OBJECTIVES: The objective of this study was to investigate the wound healing activity of Capparis ovata var. palaestina fruit extract in mice. For this aim, wound healing, in vitro antimicrobial and antioxidant activities, HPLC, phenolic and flavonoid compounds analyses were performed. METHODS: The wound healing effect was tested by excisional wound model. Wound closure was measured for 14 days and at 14th day wound healing was assessed by levels of TGF-β, VEGF, COL1A1 and angiogenesis, granulation tissue thickness, epidermal and dermal regeneration. The antioxidant activity was calculated by DPPH and ABTS free radical scavenging assays. Antimicrobial ability was evaluated by minimum inhibitory concentration and agar well diffusion tests. KEY FINDINGS: The extract indicated significant antioxidant activity while it also exhibited antimicrobial activity. Rutin was found in the extract according to HPLC study. Moreover, the extract was found to have rich phenolic and flavonoid contents. Histological evaluation showed that extract group induced significant (P < 0.001) wound healing activity compared to control group. Furthermore, extract group increased wound healing rates by promoting granulation tissue, epidermal regeneration, angiogenesis, collagen, TGF-β and VEGF. CONCLUSIONS: The results clarified that the extract possesses antioxidant, antimicrobial activity and thus could provide a valuable contribution to the wound healing.
Authors: Niloy Barua; Md Arfin Ibn Aziz; Abu Montakim Tareq; Mohammed Aktar Sayeed; Najmul Alam; Nobi Ul Alam; Mohammad Amran Uddin; Chadni Lyzu; Talha Bin Emran Journal: Biochem Biophys Rep Date: 2020-06-09