Jinmin Ma1,2,3, Fang Zhao4, Wei Su1, Qiongfang Li1,3, Jiandong Li1,3, Jingkai Ji1,3, Yong Deng4, Yang Zhou4, Xinfa Wang4, Huanming Yang1,5, Nitin K Saksena1,6, Karsten Kristiansen2, Hui Wang1,7, Yingxia Liu4. 1. BGI-Shenzhen, Shenzhen 518083, PR China. 2. Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen 2100, Denmark. 3. China National GeneBank, BGI-Shenzhen, Shenzhen 518120, PR China. 4. Shenzhen Third People's Hospital, Shenzhen 518112, PR China. 5. James D. Watson Institute of Genome Science, Hangzhou 310007, PR China. 6. IGO, 19a Boundary Street, Rushcutters Bay, Sydney, NSW, Australia. 7. Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK.
Abstract
AIM: Co-infection in HIV-1 patients with Mycobacterium tuberculosis poses considerable risk of developing the immune reconstitution inflammatory syndrome (IRIS), especially upon the initiation of antiretroviral therapy (ART). Methodology & results: For transcriptomic analysis, peripheral blood mononuclear cells' whole gene expression was used from three patient groups: HIV+ (H), HIV-TB+ (HT), HIV-TB+ with IRIS (HTI). Pathway enrichment and functional analysis was performed before and after highly active ART. Genes in the interferon-stimulating and ZNF families maintained tight functional interaction and tilted the balance in favor of TB-IRIS. DISCUSSION & CONCLUSION: The functional impairment of interaction between ZNF genes and interferon-stimulated genes, along with higher expression of S100A8/S100A9 genes possibly forms the genomic basis of TB-IRIS in a subset of HIV patients while on highly active ART.
AIM: Co-infection in HIV-1patients with Mycobacterium tuberculosis poses considerable risk of developing the immune reconstitution inflammatory syndrome (IRIS), especially upon the initiation of antiretroviral therapy (ART). Methodology & results: For transcriptomic analysis, peripheral blood mononuclear cells' whole gene expression was used from three patient groups: HIV+ (H), HIV-TB+ (HT), HIV-TB+ with IRIS (HTI). Pathway enrichment and functional analysis was performed before and after highly active ART. Genes in the interferon-stimulating and ZNF families maintained tight functional interaction and tilted the balance in favor of TB-IRIS. DISCUSSION & CONCLUSION: The functional impairment of interaction between ZNF genes and interferon-stimulated genes, along with higher expression of S100A8/S100A9 genes possibly forms the genomic basis of TB-IRIS in a subset of HIV patients while on highly active ART.
Authors: Carson M Quinn; Victoria Poplin; John Kasibante; Kyle Yuquimpo; Jane Gakuru; Fiona V Cresswell; Nathan C Bahr Journal: Life (Basel) Date: 2020-10-29