Literature DB >> 2998342

A simple one-step procedure for the separation of calpain I, calpain II and calpastatin.

J O Karlsson, S Gustavsson, C Hall, E Nilsson.   

Abstract

The soluble fraction from rabbit brain was adsorbed on a column of phenyl-Sepharose. By applying a linear gradient with decreasing salt concentration and increasing pH, it was possible to separate calpain I and calpain II from each other and from the endogenous inhibitor calpastatin. Both enzymes were capable of degrading endogenously labelled neuronal proteins, including slowly axonally transported soluble proteins and rapidly transported membrane-bound proteins, as well as casein.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 2998342      PMCID： PMC1152724          DOI： 10.1042/bj2310201

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  A NEUTRAL, CALCIUM-ACTIVATED PROTEINASE FROM THE SOLUBLE FRACTION OF RAT BRAIN.

Authors:  G GUROFF
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

2.  Use of fluorescamine in the chromatographic analysis of peptides from proteins.

Authors:  N Nakai; C Y Lai; B L Horecker
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

3.  Nerve terminal proteins of the rabbit visual relay nuclei identified by axonal transport and two-dimensional gel electrophoresis.

Authors:  J A Wagner; A S Kelly; R B Kelly
Journal:  Brain Res       Date:  1979-05-18       Impact factor: 3.252

4.  Intracellular Ca2+-dependent protease (calpain) and its high-molecular-weight endogenous inhibitor (calpastatin).

Authors:  T Murachi; K Tanaka; M Hatanaka; T Murakami
Journal:  Adv Enzyme Regul       Date:  1980

5.  A calcium-activated protease possibly involved in myofibrillar protein turnover. Isolation of a low-calcium-requiring form of the protease.

Authors:  W R Dayton; J V Schollmeyer; R A Lepley; L R Cortés
Journal:  Biochim Biophys Acta       Date:  1981-05-14

6.  Purification and characterization of a calcium-activated neutral protease from rabbit skeletal muscle which requires calcium ions of microM order concentration.

Authors:  M Inomata; M Hayashi; M Nakamura; K Imahori; S Kawashima
Journal:  J Biochem       Date:  1983-01       Impact factor: 3.387

7.  Canine cardiac calcium-dependent proteases: Resolution of two forms with different requirements for calcium.

Authors:  R L Mellgren
Journal:  FEBS Lett       Date:  1980-01-01       Impact factor: 4.124

8.  Two-dimensional gel electrophoresis of proteins in rapid axoplasmic transport.

Authors:  G C Stone; D L Wilson; M E Hall
Journal:  Brain Res       Date:  1978-04-14       Impact factor: 3.252

9.  Purification and characterization of two forms of Ca2+-activated neutral protease from calf brain.

Authors:  M N Malik; M D Fenko; K Iqbal; H M Wisniewski
Journal:  J Biol Chem       Date:  1983-07-25       Impact factor: 5.157

10.  Two distinct Ca2+ proteases (calpain I and calpain II) purified concurrently by the same method from rat kidney.

Authors:  N Yoshimura; T Kikuchi; T Sasaki; A Kitahara; M Hatanaka; T Murachi
Journal:  J Biol Chem       Date:  1983-07-25       Impact factor: 5.157
View more
  8 in total

1.  Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division.

Authors:  J S Arthur; J S Elce; C Hegadorn; K Williams; P A Greer
Journal:  Mol Cell Biol       Date:  2000-06       Impact factor: 4.272

2.  Developmental changes of calpain and calpastatin in rabbit brain.

Authors:  K Blomgren; J O Karlsson
Journal:  Neurochem Res       Date:  1989-11       Impact factor: 3.996

3.  A possible physiological role of the Ca-dependent protease calpain and its inhibitor calpastatin on the Ca current in guinea pig myocytes.

Authors:  B Belles; J Hescheler; W Trautwein; K Blomgren; J O Karlsson
Journal:  Pflugers Arch       Date:  1988-10       Impact factor: 3.657

4.  Calpain I remains intact and intracellular during platelet activation. Immunochemical measurements with monoclonal and polyclonal antibodies.

Authors:  J A Samis; G Zboril; J S Elce
Journal:  Biochem J       Date:  1987-09-01       Impact factor: 3.857

5.  Action of calpastatin in prevention of cardiac L-type Ca2+ channel run-down cannot be mimicked by synthetic calpain inhibitors.

Authors:  K Seydl; J O Karlsson; A Dominik; H Gruber; C Romanin
Journal:  Pflugers Arch       Date:  1995-02       Impact factor: 3.657

6.  Calpain activity in adult and aged human brain regions.

Authors:  M Banay-Schwartz; T DeGuzman; M Palkovits; A Lajtha
Journal:  Neurochem Res       Date:  1994-05       Impact factor: 3.996

7.  Alteration of protease levels in different brain areas of suicide victims.

Authors:  M Banay-Schwartz; T DeGuzman; G Faludi; A Lajtha; M Palkovits
Journal:  Neurochem Res       Date:  1998-07       Impact factor: 3.996

8.  Calpain processing of brain microtubules from the Atlantic cod, Gadus morhua.

Authors:  M Billger; E Nilsson; J O Karlsson; M Wallin
Journal:  Mol Cell Biochem       Date:  1993-04-07       Impact factor: 3.396
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.