G Hazirolan1, N Koçak2, A Karagöz3. 1. Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Hacettepe Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji ABD, Sıhhiye, 06100 Ankara, Turkey. Electronic address: drgulsencetin@yahoo.com. 2. Selçuk Üniversity Medicine Faculty, Department of Medical Genetics, Selçuk Üniversitesi Tıp Fakültesi Tıbbi Genetik ABD Selçuklu/Konya, Ankara, Turkey. 3. Public Health Agency of Turkey, National Molecular Microbiology Reference Centers Laboratory, Türkiye Halk Sağlığı Kurumu, Moleküler Mikrobiyoloji Referans Laboratuvarları, Adnan Saygun Cad. No: 55 Sıhhiye, Ankara, Turkey.
Abstract
INTRODUCTION: Trichosporon asahii is the most common species that causes trichosporonosis. MATERIALS AND METHODS: In the present study, a collection of 68T. asahii strains recovered from hospitalized patients urine samples between 2011 and 2016 was examined. T. asahii strains were identified by sequencing the intergenic spacer 1 region (IGS1) and genotyped. In addition, proteinase, phospholipase, esterase, haemolytic activity, and biofilm formation of a total of T. asahii strains were investigated. RESULTS: The predominant genotype was 1 (79.3%) and followed by 5 (8%), 3 (6.9%), 6 (3.4%), 4 (1.1%), 9 (1.1%). In none of the 68 strains, proteinase and phospholipase activities could be detected, while all were found to be esterase positive. Biofilm production and hemolytic activity were detected in 23.5 and 97% respectively. DISCUSSION: Our results indicated that six genotypes were (1, 5, 3, 6, 4, 9) present among T. asahii strains and no property was found to associate with a genotype, in terms of virulence factors.
INTRODUCTION:Trichosporon asahii is the most common species that causes trichosporonosis. MATERIALS AND METHODS: In the present study, a collection of 68T. asahii strains recovered from hospitalized patients urine samples between 2011 and 2016 was examined. T. asahii strains were identified by sequencing the intergenic spacer 1 region (IGS1) and genotyped. In addition, proteinase, phospholipase, esterase, haemolytic activity, and biofilm formation of a total of T. asahii strains were investigated. RESULTS: The predominant genotype was 1 (79.3%) and followed by 5 (8%), 3 (6.9%), 6 (3.4%), 4 (1.1%), 9 (1.1%). In none of the 68 strains, proteinase and phospholipase activities could be detected, while all were found to be esterase positive. Biofilm production and hemolytic activity were detected in 23.5 and 97% respectively. DISCUSSION: Our results indicated that six genotypes were (1, 5, 3, 6, 4, 9) present among T. asahii strains and no property was found to associate with a genotype, in terms of virulence factors.
Authors: Elaine Patrícia Tavares do Espírito Santo; Ruan Campos Monteiro; Ana Roberta Fusco da Costa; Silvia Helena Marques-da-Silva Journal: Mycopathologia Date: 2019-11-27 Impact factor: 2.574
Authors: Elaine Cristina Francisco; João N de Almeida Junior; Flávio Queiroz-Telles; Valério Rodrigues Aquino; Ana Verena A Mendes; Marcio de Oliveira Silva; Paulo de Tarso O E Castro; Thais Guimarães; Vinicius Ponzio; Rosane C Hahn; Guilherme M Chaves; Arnaldo L Colombo Journal: Antimicrob Agents Chemother Date: 2021-02-17 Impact factor: 5.191