Metagenomic binning reconstruction coupled with automatic pipeline annotation and giant viruses: A potential source of mistake in annotations.

Metagenomic binning reconstructions represent an emergent powerful tool to discover novel microbial genomes and explore the diversity in microbial communities. This method is sometimes coupled with automatic pipeline to perform automatic annotations. Nevertheless, we found a publish Alphaproteobacteria in public database that containing 20 contigs identified as a bacterium that were actually a novel giant viruses close to Aureococcus anophagefferens virus. This virus was completely misidentified by automatic pipeline and missed by control program and finally concern near to 20% of this 2,4 Mb.