Roopa G Manjunatha1, Sunil Sharma2, Ravi Prakash Narayan2, Veena Koul1. 1. Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New-Delhi, India. 2. Department of Burns and Plastic Surgery, Vardhman Mahavir Medical College & Safdarjung Hospital, New-Delhi, India.
Abstract
BACKGROUND: Lidocaine Hydrochloride (HCL) is one of the commonest topical local anesthetic drugs. The permeation of the lidocaine can be enhanced by iontophoresis (IOP). The purpose of this study was to evaluate the permeability of 2.5 and 5% lidocaine permeation in ex vivo human skin using different IOP waveform. METHODS: Continuous and modulated IOP at the current density of 0.5 mA/cm2 were applied across human skin (n = 3) in donor chamber of vertical diffusion cell at 2.5 and 5% lidocaine concentration. High Performance Liquid Chromatography was used to determine lidocaine concentration. RESULTS: Findings revealed that lidocaine concentration increased effectively in a time-dependent manner in both modulated and continuous IOP at 2.5 and 5% lidocaine concentration. Compared to the passive group, the flux of lidocaine with modulated and continuous IOP were higher of about six and ten-fold, respectively. However, no significant difference was observed between continuous and modulated IOP groups at both lidocaine concentrations. At 2.5% lidocaine concentration, the permeation time taken by modulated and continuous IOP to attain therapeutic levels of 142 and 164 μg/cm2 , respectively, was approximately 10 minutes. At 5% lidocaine, the therapeutic permeation of 129 and 147 μg/cm2 , respectively, was achieved at approximately 5 minutes after applying iontophoresis waveform. CONCLUSION: Study shows that modulated IOP can be a promising alternative method in clinical settings aside from continuous IOP. Based on the clinical requirements, IOP can be used at 2.5 and 5% lidocaine concentration depending on need of relatively short or very short onset action.
BACKGROUND:Lidocaine Hydrochloride (HCL) is one of the commonest topical local anesthetic drugs. The permeation of the lidocaine can be enhanced by iontophoresis (IOP). The purpose of this study was to evaluate the permeability of 2.5 and 5% lidocaine permeation in ex vivo human skin using different IOP waveform. METHODS: Continuous and modulated IOP at the current density of 0.5 mA/cm2 were applied across human skin (n = 3) in donor chamber of vertical diffusion cell at 2.5 and 5% lidocaine concentration. High Performance Liquid Chromatography was used to determine lidocaine concentration. RESULTS: Findings revealed that lidocaine concentration increased effectively in a time-dependent manner in both modulated and continuous IOP at 2.5 and 5% lidocaine concentration. Compared to the passive group, the flux of lidocaine with modulated and continuous IOP were higher of about six and ten-fold, respectively. However, no significant difference was observed between continuous and modulated IOP groups at both lidocaine concentrations. At 2.5% lidocaine concentration, the permeation time taken by modulated and continuous IOP to attain therapeutic levels of 142 and 164 μg/cm2 , respectively, was approximately 10 minutes. At 5% lidocaine, the therapeutic permeation of 129 and 147 μg/cm2 , respectively, was achieved at approximately 5 minutes after applying iontophoresis waveform. CONCLUSION: Study shows that modulated IOP can be a promising alternative method in clinical settings aside from continuous IOP. Based on the clinical requirements, IOP can be used at 2.5 and 5% lidocaine concentration depending on need of relatively short or very short onset action.
Authors: Clara López-Iglesias; Cristina Quílez; Joana Barros; Diego Velasco; Carmen Alvarez-Lorenzo; José L Jorcano; Fernando J Monteiro; Carlos A García-González Journal: Pharmaceutics Date: 2020-09-12 Impact factor: 6.321