Literature DB >> 29971716

High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates.

Keith J Mickolajczyk1, William O Hancock2.   

Abstract

This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described.

Entities:  

Keywords:  Gold nanoparticle; Kinesin; Single-molecule; Total internal reflection dark-field microscopy

Mesh：

Substances：

Year:  2018        PMID： 29971716      PMCID： PMC7851763          DOI： 10.1007/978-1-4939-8556-2_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  43 in total

1.  Assembly dynamics of microtubules at molecular resolution.

Authors:  Jacob W J Kerssemakers; E Laura Munteanu; Liedewij Laan; Tim L Noetzel; Marcel E Janson; Marileen Dogterom
Journal:  Nature       Date:  2006-06-25       Impact factor: 49.962

2.  Objective-type dark-field illumination for scattering from microbeads.

Authors:  I Braslavsky; R Amit; B M Jaffar Ali; O Gileadi; A Oppenheim; J Stavans
Journal:  Appl Opt       Date:  2001-11-01       Impact factor: 1.980

3.  Kinesin's step dissected with single-motor FRET.

Authors:  Sander Verbrugge; Zdenek Lansky; Erwin J G Peterman
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-01       Impact factor: 11.205

4.  Interferometric scattering microscopy (iSCAT): new frontiers in ultrafast and ultrasensitive optical microscopy.

Authors:  Jaime Ortega-Arroyo; Philipp Kukura
Journal:  Phys Chem Chem Phys       Date:  2012-09-20       Impact factor: 3.676

Review 5.  Total internal reflection fluorescence.

Authors:  D Axelrod; T P Burghardt; N L Thompson
Journal:  Annu Rev Biophys Bioeng       Date:  1984

6.  Processivity of the motor protein kinesin requires two heads.

Authors:  W O Hancock; J Howard
Journal:  J Cell Biol       Date:  1998-03-23       Impact factor: 10.539

7.  Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization.

Authors:  Ahmet Yildiz; Joseph N Forkey; Sean A McKinney; Taekjip Ha; Yale E Goldman; Paul R Selvin
Journal:  Science       Date:  2003-06-05       Impact factor: 47.728

8.  Surface-bound casein modulates the adsorption and activity of kinesin on SiO2 surfaces.

Authors:  Tomomitsu Ozeki; Vivek Verma; Maruti Uppalapati; Yukiko Suzuki; Mikihiko Nakamura; Jeffrey M Catchmark; William O Hancock
Journal:  Biophys J       Date:  2009-04-22       Impact factor: 4.033

9.  Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.

Authors:  D D Hackney
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

10.  Molecular counting by photobleaching in protein complexes with many subunits: best practices and application to the cellulose synthesis complex.

Authors:  Yalei Chen; Nathan C Deffenbaugh; Charles T Anderson; William O Hancock
Journal:  Mol Biol Cell       Date:  2014-09-17       Impact factor: 3.612
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  2 in total

1.  Insights into Kinesin-1 Stepping from Simulations and Tracking of Gold Nanoparticle-Labeled Motors.

Authors:  Keith J Mickolajczyk; Annan S I Cook; Janak P Jevtha; John Fricks; William O Hancock
Journal:  Biophys J       Date:  2019-06-20       Impact factor: 4.033

Review 2.  Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles.

Authors:  Ralf P Friedrich; Mona Kappes; Iwona Cicha; Rainer Tietze; Christian Braun; Regine Schneider-Stock; Roland Nagy; Christoph Alexiou; Christina Janko
Journal:  Int J Nanomedicine       Date:  2022-05-13
  2 in total

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