Ching-Lung Liao1, Yung Lin Chu2, Hui-Yi Lin3, Cheng-Yen Chen4, Ming-Jie Hsu4, Kuo-Ching Liu5, Kuang-Chi Lai6,7, An-Cheng Huang8, Jing-Gung Chung9,10. 1. College of Chinese Medicine, School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. 2. International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C. 3. School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C. 4. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 5. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C. 6. Department of Medical Laboratory Science and Biotechnology, College of Medicine and Life Science, Chung Hwa University of Medical Technology, Tainan, Taiwan, R.O.C. 7. Department of Surgery, China Medical University Beigang Hospital, Beigang, Taiwan, R.O.C. 8. Department of Nursing, St. Mary's Junior College of Medicine, Nursing and Management, Yilan, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw haj@smc.edu.tw. 9. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw haj@smc.edu.tw. 10. Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
Abstract
BACKGROUND/AIM: Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells. MATERIALS AND METHODS: Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro. RESULTS: BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERK1/2), E-cadherin and nuclear factor-ĸB (NF-ĸB) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-ĸB when compared to controls. CONCLUSION: BDMC may have potential as a novel anti-metastasis agent for the treatment of human cervical cancer. Copyright
BACKGROUND/AIM: Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells. MATERIALS AND METHODS: Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro. RESULTS: BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERK1/2), E-cadherin and nuclear factor-ĸB (NF-ĸB) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-ĸB when compared to controls. CONCLUSION: BDMC may have potential as a novel anti-metastasis agent for the treatment of human cervical cancer. Copyright
Authors: Natalia Miklášová; Peter Herich; Juan Carlos Dávila-Becerril; Joaquín Barroso-Flores; Eva Fischer-Fodor; Jindra Valentová; Janka Leskovská; Jozef Kožíšek; Peter Takáč; Ján Mojžiš Journal: Molecules Date: 2021-07-20 Impact factor: 4.411