Mariusz Chabowski1,2, Aleksandra Nowak3, Jedrzej Grzegrzolka3, Aleksandra Piotrowska3, Dariusz Janczak4,2, Piotr Dziegiel3. 1. Division of Surgical Specialties, Department of Clinical Nursing, Faculty of Health Science, 4th Military Teaching Hospital, Wroclaw, Poland; Wroclaw Medical University, Wroclaw, Poland mariusz.chabowski@gmail.com. 2. Department of Surgery, 4th Military Teaching Hospital, Wroclaw, Poland; Wroclaw Medical University, Wroclaw, Poland. 3. Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, Wroclaw, Poland. 4. Division of Surgical Specialties, Department of Clinical Nursing, Faculty of Health Science, 4th Military Teaching Hospital, Wroclaw, Poland; Wroclaw Medical University, Wroclaw, Poland.
Abstract
BACKGROUND/AIM: The aim of the present study was to compare the prognostic value of nestin-positive microvessel density (nestin+ MVD) to the CD34-positive (CD34+) MVD in patients with colorectal cancer. MATERIALS AND METHODS: A total of 125 cases of colorectal cancer were used for the study. Immunohistochemical reactions were performed on paraffin sections using anti-nestin and anti-CD34. The average MVD was determined for both markers and was referenced to patients' clinicopathological data. RESULTS: A strong positive correlation was found between the MVDs for both analyzed antigens. Contrary to CD34+ MVD, a positive trend was observed between high nestin+ MVD and lymph node invasion. Moreover, significantly higher nestin+ MVD was noted in patients with more advanced clinical stage than in those with early-stage cancer. No such correlation was found for CD34 antigen. Additionally, a possible trend between the higher nestin+ MVD and shorter event-free survival was noted, whereas no such relation was observed for CD34+ MVD. CONCLUSION: The results suggest that nestin might be a reliable marker for angiogenesis evaluation in patients with colorectal cancer. In contrast to CD34, the expression of nestin in blood vessels seems to be a more sensitive marker of cancer progression. Copyright
BACKGROUND/AIM: The aim of the present study was to compare the prognostic value of nestin-positive microvessel density (nestin+ MVD) to the CD34-positive (CD34+) MVD in patients with colorectal cancer. MATERIALS AND METHODS: A total of 125 cases of colorectal cancer were used for the study. Immunohistochemical reactions were performed on paraffin sections using anti-nestin and anti-CD34. The average MVD was determined for both markers and was referenced to patients' clinicopathological data. RESULTS: A strong positive correlation was found between the MVDs for both analyzed antigens. Contrary to CD34+ MVD, a positive trend was observed between high nestin+ MVD and lymph node invasion. Moreover, significantly higher nestin+ MVD was noted in patients with more advanced clinical stage than in those with early-stage cancer. No such correlation was found for CD34 antigen. Additionally, a possible trend between the higher nestin+ MVD and shorter event-free survival was noted, whereas no such relation was observed for CD34+ MVD. CONCLUSION: The results suggest that nestin might be a reliable marker for angiogenesis evaluation in patients with colorectal cancer. In contrast to CD34, the expression of nestin in blood vessels seems to be a more sensitive marker of cancer progression. Copyright