Seongmin Kim1, Sung Gyu Park2,3, Yoon Jae Song2, Jeong Koo Park3, Cheol Hee Choi4, Sanghoon Lee5, Robert M Hoffman6,7. 1. Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea. 2. Department of Life Science, Gachon University, Seongnam, Republic of Korea. 3. Metabio Inc., Seoul, Republic of Korea. 4. Department of Pharmacology, Chosun University Medical School, Gwangju, Republic of Korea. 5. Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea mdleesh@gmail.com. 6. AntiCancer Inc., San Diego, CA, U.S.A. 7. Department of Surgery, University of California, San Diego, CA, U.S.A.
Abstract
BACKGROUND/AIM: Dendropanax morbifera (DM) and Commersonia bartramia (CB) are possible candidates for immunotherapy. In this study, the cytotoxicity and chemical sensitization of DM and CB extracts on gynecologic and colon cancers were evaluated. MATERIALS AND METHODS: The malignant cell lines were cultured and analyzed for cytotoxicity and chemical sensitization. A mouse model was also constructed to make the condition similar to in vivo. Reverse transcription-polymerase chain reaction was conducted to determine alterations in drug-resistant genes. RESULTS: The extracts from DM and CB showed specific cytotoxicity to malignant cell lines. DM increased chemical sensitivity to cervical and ovarian cancer, while CB showed improved sensitization to endometrial cancer. The effects of the extracts were confirmed using a mouse model. The extracts induced differences in the expression levels of a number of genes related to drug resistance. CONCLUSION: DM and CB extracts could be novel agents for immunotherapy and chemical sensitization in gynecologic and colon cancers. Copyright
BACKGROUND/AIM: Dendropanax morbifera (DM) and Commersonia bartramia (CB) are possible candidates for immunotherapy. In this study, the cytotoxicity and chemical sensitization of DM and CB extracts on gynecologic and colon cancers were evaluated. MATERIALS AND METHODS: The malignant cell lines were cultured and analyzed for cytotoxicity and chemical sensitization. A mouse model was also constructed to make the condition similar to in vivo. Reverse transcription-polymerase chain reaction was conducted to determine alterations in drug-resistant genes. RESULTS: The extracts from DM and CB showed specific cytotoxicity to malignant cell lines. DM increased chemical sensitivity to cervical and ovarian cancer, while CB showed improved sensitization to endometrial cancer. The effects of the extracts were confirmed using a mouse model. The extracts induced differences in the expression levels of a number of genes related to drug resistance. CONCLUSION:DM and CB extracts could be novel agents for immunotherapy and chemical sensitization in gynecologic and colon cancers. Copyright