Identification and characterization of 23 microsatellite loci for Chlorostoma rustica based on RAD-seq.

Chlorostoma rustica is an ecologically and economically important species in China, which plays an important role on the overall function of the coastal systems. Understanding of the genetic structure of C. rustica populations is vital to breeding strategies and conservation programmes. In this study, we isolated and characterized 23 microsatellite loci with high polymorphism using the restriction site-associated DNA sequencing approach. The number of alleles per locus ranged from 6 to 18, and the expected heterozygosities varied from 0. 760 to 0.936. All the polymorphism information content values of the 23 loci were greater than 0.5, indicating that these markers were highly informative and laid the foundation for further genetic analysis.