Literature DB >> 29966501

Immunohistochemistry in Investigative and Toxicologic Pathology.

Kyathanahalli S Janardhan1, Heather Jensen2, Natasha P Clayton2, Ronald A Herbert2.   

Abstract

Immunohistochemistry (IHC) is a valuable tool in pathology. This review provides a brief description of the technical aspects of IHC and a detailed discussion on the variables that affect the results, interpretation, and reproducibility of IHC results. Lists of antibodies that have and have not worked in IHC on various mouse and rat tissues in our laboratory are provided as a guidance for selection of antibodies. An approach to IHC method optimization is presented. Finally, the critical information that should be included as a part of peer-reviewed manuscript is also discussed.

Entities:  

Keywords:  IHC; antibodies; controls; fixation; mice; rat; reproducibility

Mesh：

Substances：

Year:  2018        PMID： 29966501      PMCID： PMC6033701          DOI： 10.1177/0192623318776907

Source DB:  PubMed          Journal:  Toxicol Pathol        ISSN： 0192-6233            Impact factor:   1.902


  48 in total

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Journal:  Vet Pathol       Date:  2013-09-27       Impact factor: 2.221

4.  Reproducibility crisis: Blame it on the antibodies.

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Journal:  Nature       Date:  2015-05-21       Impact factor: 49.962

5.  Signal amplification in immunohistochemistry: loose-jointed deformable heteropolymeric HRP conjugates vs. linear polymer backbone HRP conjugates.

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Journal:  Acta Histochem       Date:  2013-01-23       Impact factor: 2.479

6.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

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7.  Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections.

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Journal:  Lab Invest       Date:  1975-07       Impact factor: 5.662

Review 8.  Review: application of monoclonal antibody techniques to biochemical research.

Authors:  G S Eisenbarth
Journal:  Anal Biochem       Date:  1981-02       Impact factor: 3.365

9.  Controls for immunohistochemistry: the Histochemical Society's standards of practice for validation of immunohistochemical assays.

Authors:  Stephen M Hewitt; Denis G Baskin; Charles W Frevert; William L Stahl; Eduardo Rosa-Molinar
Journal:  J Histochem Cytochem       Date:  2014-07-14       Impact factor: 2.479

Review 10.  Tissue fixation and the effect of molecular fixatives on downstream staining procedures.

Authors:  William J Howat; Beverley A Wilson
Journal:  Methods       Date:  2014-02-21       Impact factor: 3.608
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  8 in total

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Review 5.  Identification of technology frontiers of artificial intelligence-assisted pathology based on patent citation network.

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Review 6.  Immunohistochemistry as a detection tool for ion channels involved in dental pain signaling.

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7.  Polyclonal antibody-based immunohistochemical detection of intraleukocytic Theileria parasites in roan and sable antelopes.

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Review 8.  Histopathologic Evaluation and Scoring of Viral Lung Infection.

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Journal:  Methods Mol Biol       Date:  2020
  8 in total

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