| Literature DB >> 2996425 |
J M van der Vossen, J Kok, G Venema.
Abstract
Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.Entities:
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Year: 1985 PMID: 2996425 PMCID: PMC238658 DOI: 10.1128/aem.50.2.540-542.1985
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792