Literature DB >> 2996025

Characterization of recombinant monkey and human erythropoietin.

J C Egrie, J Browne, P Lai, F K Lin.   

Abstract

We have isolated the human EPO gene from a human genomic lambda library and a monkey EPO cDNA from an anemic monkey kidney cDNA library. These sequences have been expressed in both COS1 and Chinese hamster ovary cells. The expressed EPO has been shown to be fully biologically active both in vitro and in vivo and immunologically identical to the native hormone. In addition, administration of recombinant EPO to animals causes a dramatic increase in their hematocrit.

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Year:  1985        PMID： 2996025

Source DB:  PubMed          Journal:  Prog Clin Biol Res        ISSN： 0361-7742


  7 in total

1.  Presence and transcription of intracisternal A-particle-related sequences in CHO cells.

Authors:  K P Anderson; Y S Lie; M A Low; S R Williams; E H Fennie; T P Nguyen; F M Wurm
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

2.  Erythropoietin crosses the blood-brain barrier to protect against experimental brain injury.

Authors:  M L Brines; P Ghezzi; S Keenan; D Agnello; N C de Lanerolle; C Cerami; L M Itri; A Cerami
Journal:  Proc Natl Acad Sci U S A       Date:  2000-09-12       Impact factor: 11.205

3.  Chinese hamster ovary cells contain transcriptionally active full-length type C proviruses.

Authors:  Y S Lie; E M Penuel; M A Low; T P Nguyen; J O Mangahas; K P Anderson; C J Petropoulos
Journal:  J Virol       Date:  1994-12       Impact factor: 5.103

4.  Erythropoietin reduces myocardial infarction and left ventricular functional decline after coronary artery ligation in rats.

Authors:  Chanil Moon; Melissa Krawczyk; Dongchoon Ahn; Ismayil Ahmet; Doojin Paik; Edward G Lakatta; Mark I Talan
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-19       Impact factor: 11.205

5.  Human erythropoietin gene: high level expression in stably transfected mammalian cells and chromosome localization.

Authors:  J S Powell; K L Berkner; R V Lebo; J W Adamson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

6.  Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.

Authors:  G Migliaccio; A R Migliaccio; B L Kreider; G Rovera; J W Adamson
Journal:  J Cell Biol       Date:  1989-08       Impact factor: 10.539

7.  Erythropoiesis stimulating agents: approaches to modulate activity.

Authors:  Angus M Sinclair
Journal:  Biologics       Date:  2013-07-03
  7 in total

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