Bovine Granulosa Cell Culture.

Culture of granulosa cells has for long provided a useful tool to understand the molecular processes underlying ovarian follicle development. Among all species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we describe up-to-date methods for the harvesting of granulosa cells from bovine ovaries collected post-mortem, as well as procedures for both culturing granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro.